AI Article Synopsis
- This study analyzed the genetic diversity and origins of Daweishan Mini chickens using mitochondrial DNA (mtDNA) sequencing.
- Blood samples from 30 individual chickens were collected, and a specific region of their DNA (the D-loop) was amplified and sequenced.
- The findings revealed six distinct haplotypes clustered into four main groups, with two dominant clades indicating a likely evolutionary link to the Red Junglefowl subspecies G.g. spadiceus.
Article Abstract
This study evaluated the genetic diversity and origin of Daweishan Mini chickens using mtDNA sequence polymorphism. Blood samples from 30 Daweishan Mini chickens were collected. The complete D-loop was PCR amplified, sequenced and compared with the DNA data of five Red Junglefowl (Gallus gallus) subspecies. Eighteen variable sites that defined six haplotypes were observed. The six haplotypes were clustered into four clades (A, B, D and E), of which clade A and B were dominant. Clades Aand B were clustered with G.g. spadiceus, indicating these two clades may have originated from this subspecies. These results show there is diversity in the middle of the mtDNA D-loop, and indicate there are multiple maternal origins for Daweishan Mini chickens. It appears that G.g. spadiceus contributed more to the evolution of the Daweishan Mini chickens breed than the other four subspecies tested here.
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| http://dx.doi.org/10.3109/19401736.2015.1060478 | DOI Listing |
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