Horne-Badovinac tracks how coordinated cellular movements mold tissues.
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http://dx.doi.org/10.1083/jcb.2101pi | DOI Listing |
Mol Biol Cell
January 2024
Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637.
The Hippo pathway is an evolutionarily conserved regulator of tissue growth. Multiple Hippo signaling components are regulated via proteolytic degradation. However, how these degradation mechanisms are themselves modulated remains unexplored.
View Article and Find Full Text PDFJ Cell Sci
March 2024
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA.
Migrating epithelial cells globally align their migration machinery to achieve tissue-level movement. Biochemical signaling across leading-trailing cell-cell interfaces can promote this alignment by partitioning migratory behaviors like protrusion and retraction to opposite sides of the interface. However, how signaling proteins become organized at interfaces to accomplish this is poorly understood.
View Article and Find Full Text PDFbioRxiv
March 2023
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA.
Migrating epithelial cells globally align their migration machinery to achieve tissue-level movement. Biochemical signaling across leading-trailing cell-cell interfaces can promote this alignment by partitioning migratory behaviors like protrusion and retraction to opposite sides of the interface. However, how the necessary signaling proteins become organized at this site is poorly understood.
View Article and Find Full Text PDFMethods Mol Biol
February 2023
Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL, USA.
The Drosophila egg chamber is a powerful system to study epithelial cell collective migration and polarized basement membrane secretion. A strength of this system is the ability to capture these dynamic processes in ex vivo organ culture using high-resolution live imaging. Ex vivo culture also allows acute pharmacological or labeling treatments, extending the versatility of the system.
View Article and Find Full Text PDFMethods Mol Biol
February 2023
Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL, USA.
The follicular epithelial cells of the Drosophila egg chamber have become a premier model to study how cells globally orient their actin-based machinery for collective migration. The basal surface of each follicle cell has lamellipodial and filopodial protrusions that extend from its leading edge and an array of stress fibers that mediate its adhesion to the extracellular matrix; these migratory structures are all globally aligned in the direction of tissue movement. To understand how this global alignment is achieved, one must be able to reliably visualize the underlying F-actin; however, dynamic F-actin networks can be difficult to preserve in fixed tissues.
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