An integrated capillary scale (300 μm id) ceramic microfluidic LC system combined with MS/MS has been successfully employed for the quantitative analysis of pharmaceutical compounds in human plasma. The capillary ceramic microfluidic LC/MS/MS system showed an approximate 20-fold (range 11-38-fold) increase in sensitivity compared with a standard 2.1 mm scale UPLC/MS/MS system for a broad range of analytes. The loading capacity of the devices capillary separations channel allowed injection of 2 μL of an aqueous solution, and up to 1.2 μL of a typical protein-precipitated plasma sample, onto the reversed-phase chromatography system. The system also showed excellent chromatographic performance and robustness, with no deleterious effects on the chromatography observed over the course of 1000 injections of protein-precipitated plasma. The ability of the ceramic microfluidic LC/MS/MS system to deliver this level of sensitivity and performance enables the routine quantification of pharmaceutical compounds from small format samples, such as those obtained by dried blood spot or other blood microsampling approaches, to be performed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c5an00646e | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment.
Bioact Mater
March 2025
College of Biomedical Engineering, Sichuan University, Chengdu, Sichuan, 610064, China.
Bioactive ceramics have been used in bone tissue repair and regeneration. However, because of the complex in vivo osteogenesis process, long cycle, and difficulty of accurately tracking, the mechanism of interaction between materials and cells has yet to be fully understood, hindering its development. The ceramic microbridge microfluidic chip system may solve the problem and provide an in vitro method to simulate the microenvironment in vivo.
View Article and Find Full Text PDFMicrofluidic-based fluorescence enhancement of silica-embedded carbon dots for direct detection and quantification of unamplified HCV RNA in clinical samples.
Anal Chim Acta
January 2025
Center of Genomics, Helmy Institute, Zewail City of Science and Technology, Sheikh Zayed Dist, 12588, Giza, Egypt; Pathology and Molecular Genomics Unit of Medical Ain Shams Research Institute (MASRI), Faculty of Medicine, Ain Shams University. Cairo, 11591, Cairo, Egypt; Biochemistry Department, Faculty of Pharmacy, Misr University for Science and Technology, 12566, Giza, Egypt. Electronic address:
Article Synopsis
- Hepatitis C Virus (HCV) is a serious, chronic infection that often shows no symptoms, making early detection crucial for treatment and infection control.
- Current detection methods like PCR are expensive and time-consuming, which limits their use in smaller labs and field settings.
- A new technology using crosslinked Enhanced Emission (CEE) has been developed for detecting HCV RNA with high sensitivity and speed, allowing results in under 20 minutes via a 3D-printed microfluidic chip, achieving impressive sensitivity (96.47%) and specificity (98.79%).
Copper-Consuming Nanoplatform for Alleviating Hypoxia and Overcoming Resistance to Sonodynamic Therapy of Breast Cancer.
ACS Appl Mater Interfaces
October 2024
Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, P. R. China.
Sonodynamic therapy (SDT) is a promising treatment modality for breast cancer; however, its effectiveness is often impeded by the hypoxic tumor microenvironment owing to an insufficient oxygen supply in the solid tumors. To overcome this challenge, we elaborately developed a 4T1 tumor-targeted multifunctional nanoagent by integrating both dendrimer-structured copper chelating agents and organic sonosensitizers (IR820) into a biotin-modified nanoliposome via a microfluidic-assisted self-assembly. In particular, the aforementioned copper chelating agent was constructed by introducing multiple xanthate groups into a dendrimer polymer, which showed a significant selectivity for the consumption of the intracellular copper levels.
View Article and Find Full Text PDFOne-pot microfluidic fabrication of micro ceramic particles.
Nat Commun
October 2024
School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.
In the quest for miniaturization across technical disciplines, microscale ceramic blocks emerge as pivotal components, with performance critically dependent on precise scales and intricate shapes. Sharp-edged ceramic microparticles, applied from micromachining to microelectronics, require innovative fabrication techniques for high-throughput production while maintaining structural complexity and mechanical integrity. This study introduces a "one-pot microfluidic fabrication" system incorporating two device fabrication strategies, "groove & tongue" and sliding assembling, achieving an unprecedented array of microparticles with diverse, complex shapes and refined precision, outperforming traditional methods in production rate and quality.
View Article and Find Full Text PDFSilica Aerogel in Microfluidic Channels: Synthesis, Chip Integration, Mechanical Reinforcement, and Characterization.
ACS Omega
October 2024
Institute for Microsensors, -Actuators and -Systems (IMSAS), University of Bremen, Bremen 28359, Germany.
Silica aerogels are highly porous materials with unique properties such as high specific surface area, high thermal insulation, and high open porosity. These characteristics make them attractive for several applications in closed microfluidic channels such as BioMEMS, catalysis, and thermal insulation. However, aerogel-filled microchannels have not been reported in the literature yet because of the complexity of creating a process that controls the integration, shrinkage, and mechanical stability of these materials inside a closed channel.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!