A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 176

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 1034
Function: getPubMedXML

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3152
Function: GetPubMedArticleOutput_2016

File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

Immunoblotting of Antigens: Whole, Strip, and New-Line Nitrocellulose Membrane Immunoblotting Using the Chemiluminescence Technique. | LitMetric

Antigen detection is a well-known tool in the scientific world that is used by clinicians and researchers to detect specific antigens in diagnosing diseases or for other medical/environmental discoveries. Antigen detection is introduced in various forms over the past decades. These techniques are often evaluated by their sensitivity, accuracy, and ease of use. One technique that has provided many advantages over typical immunochemical staining is the use of chemiluminescence. This technique has been used in various scientific fields, anywhere from clinical diagnosis to environmental research. The emission of visible radiation by compounds once exposed to sunlight has been known for centuries and currently is the main principle for chemiluminescence. Here, we introduce three different chemiluminescence techniques that are widely used in immunodetection of antigens: (a) whole membrane chemiluminescence detection, (b) strip membrane chemiluminescence detection, and

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-4939-2718-0_10DOI Listing

Publication Analysis

Top Keywords

chemiluminescence technique
8
antigen detection
8
membrane chemiluminescence
8
chemiluminescence detection
8
chemiluminescence
6
immunoblotting antigens
4
antigens strip
4
strip new-line
4
new-line nitrocellulose
4
nitrocellulose membrane
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!