We have changed the amino acid set of the genetic code of Escherichia coli by evolving cultures capable of growing on the synthetic noncanonical amino acid L-β-(thieno[3,2-b]pyrrolyl)alanine ([3,2]Tpa) as a sole surrogate for the canonical amino acid L-tryptophan (Trp). A long-term cultivation experiment in defined synthetic media resulted in the evolution of cells capable of surviving Trp→[3,2]Tpa substitutions in their proteomes in response to the 20,899 TGG codons of the E. coli W3110 genome. These evolved bacteria with new-to-nature amino acid composition showed robust growth in the complete absence of Trp. Our experimental results illustrate an approach for the evolution of synthetic cells with alternative biochemical building blocks.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4782924 | PMC |
| http://dx.doi.org/10.1002/anie.201502868 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Deciphering the Radial Ligand Effect of Biomimetic Amino Acid toward Stable Alkaline Oxygen Evolution.
Inorg Chem
January 2025
School of Materials Science and Engineering, China University of Petroleum, Qingdao 266580, PR China.
Mismatched electron and proton transport rates impede the manifestation of effective performance of the electrocatalytic oxygen evolution reaction (OER), thereby limiting its industrial applications. Inspired by the natural protein cluster in PS-II, different organic-inorganic hybrid electrocatalysts were synthesized via a hydrothermal method. -Toluidine (PT), benzoic acid (BA), and -aminobenzoic acid (PABA) were successfully intercalated into NiFe-LDH.
View Article and Find Full Text PDFGuarding Drinking Water Safety against Harmful Algal Blooms: Could UV/Cl Treatment Be the Answer?
Environ Sci Technol
January 2025
Environmental Engineering and Science, Department of Chemical and Environmental Engineering (ChEE), University of Cincinnati, Cincinnati, Ohio 45221, United States.
Frequent and severe occurrences of harmful algal blooms increasingly threaten human health by the release of microcystins (MCs). Urgent attention is directed toward managing MCs, as evidenced by rising HAB-related do not drink/do not boil advisories due to unsafe MC levels in drinking water. UV/chlorine treatment, in which UV light is applied simultaneously with chlorine, showed early promise for effectively degrading MC-LR to values below the World Health Organization's guideline limits.
View Article and Find Full Text PDFA human monoclonal antibody isolated from Japanese encephalitis virus vaccine-vaccinated volunteer neutralizing various flaviviruses.
Front Microbiol
December 2024
School of Basic Medical Sciences, Health Science Center, Ningbo University, Ningbo, China.
Introduction: Japanese encephalitis virus (JEV) and Zika virus (ZIKV) are prevalent in over 80 countries or territories worldwide, causing hundreds of thousands of cases annually. But currently there is a lack of specific antiviral agents and effective vaccines.
Methods: In the present study, to identify human neutralizing monoclonal antibody (mAb) against JEV or/and ZIKV, we isolated ZIKV-E protein-binding B cells from the peripheral venous blood of a healthy volunteer who had received the JEV live-attenuated vaccine and performed 10× Genomics transcriptome sequencing and BCR sequencing analysis, we then obtained the V region amino acid sequences of a novel mAb LZY3412.
Calorimetric measurement of energy and nutrient stimulation of microorganisms from the continental deep subsurface.
Front Microbiol
December 2024
Department of Earth Sciences, University of Southern California, Los Angeles, CA, United States.
Microbial activity in the deep continental subsurface is difficult to measure due to low cell densities, low energy fluxes, cryptic elemental cycles and enigmatic metabolisms. Nonetheless, direct access to rare sample sites and sensitive laboratory measurements can be used to better understand the variables that govern microbial life underground. In this study, we sampled fluids from six boreholes at depths ranging from 244 m to 1,478 m below ground at the Sanford Underground Research Facility (SURF), a former goldmine in South Dakota, United States.
View Article and Find Full Text PDFInhibitory Effect of Human Anti-CA I Autoantibodies and Development of Monoclonal Antibody mAb 2B8 Targeting Carbonic Anhydrase I.
Mediators Inflamm
December 2024
Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.
Spontaneous tumor regression is a recognized phenomenon across various cancer types. Recent research emphasizes the alterations in autoantibodies against carbonic anhydrase I (CA I) (anti-CA I) levels as potential prognostic markers for various malignancies. Particularly, autoantibodies targeting CA I and II appear to induce cellular damage by inhibiting their respective protein's catalytic functions.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!