AI Article Synopsis
- MicroRNAs, particularly miR-181a-5p, are significant in the development and progression of lung cancer, which is the top cancer killer globally.
- In this study, it was found that miR-181a-5p levels are notably lower in non-small-cell lung cancer (NSCLC) tissues and cell lines, and increasing its levels inhibited the growth and spread of A549 lung cancer cells.
- The research also revealed that miR-181a-5p targets the Kras gene, reducing its expression, and suggests that enhancing miR-181a-5p could be a promising therapeutic strategy for NSCLC patients.
Article Abstract
MicroRNAs play important roles in carcinogenesis and tumor progress. Lung cancer is the leading cause of cancer mortality worldwide. In this study, the function of miR-181a-5p was investigated in non-small-cell lung cancer (NSCLC). Results showed that miR-181a-5p was significantly decreased in NSCLC tissues and cell lines. The proliferation and migration of A549 cells transfected with miR-181a-5p mimic was significantly inhibited. Luciferase activity assay results demonstrated that two binding sites of Kras could be directly targeted by miR-181a-5p. Furthermore, Kras was down-regulated by miR-181a-5p at both transcriptional and translational levels. SiRNA-mediated Kras down-regulation could mimic the effects of miR-181a-5p mimic in A549 cells. Our findings suggest that miR-181a-5p plays a potential role in tumor suppression by partially targeting Kras and has the potential therapeutic application in NSCLC patients.
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| http://dx.doi.org/10.1093/abbs/gmv054 | DOI Listing |
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