Antioxidant Capacity: Experimental Determination by EPR Spectroscopy and Mathematical Modeling.

J Agric Food Chem

†Department of Materials Chemistry and Chemical Technology and ‡Department of Physical Chemistry, Institute of Chemistry, University of Silesia, Szkolna 9, 40-006 Katowice, Poland.

Published: July 2015

AI Article Synopsis

  • A new mathematical model has been developed to measure the antioxidant capacity of various food products using electron paramagnetic resonance (EPR) spectroscopy, analyzed through 1000 data points.
  • The model can determine Trolox equivalent antioxidant capacity (TEAC) values from just one EPR measurement, effectively working with a wide range of antioxidant strengths (TEAC values from 21 to 2347 μmol TE/100 mL).
  • The reliability of this new method is high, with an R² value of 0.98 comparing experimental versus calculated TEAC values, making it a fast, accurate, inexpensive, and straightforward alternative to traditional EPR methods.

Article Abstract

A new method of determining antioxidant capacity based on a mathematical model is presented in this paper. The model was fitted to 1000 data points of electron paramagnetic resonance (EPR) spectroscopy measurements of various food product samples such as tea, wine, juice, and herbs with Trolox equivalent antioxidant capacity (TEAC) values from 20 to 2000 μmol TE/100 mL. The proposed mathematical equation allows for a determination of TEAC of food products based on a single EPR spectroscopy measurement. The model was tested on the basis of 80 EPR spectroscopy measurements of herbs, tea, coffee, and juice samples. The proposed model works for both strong and weak antioxidants (TEAC values from 21 to 2347 μmol TE/100 mL). The determination coefficient between TEAC values obtained experimentally and TEAC values calculated with proposed mathematical equation was found to be R(2) = 0.98. Therefore, the proposed new method of TEAC determination based on a mathematical model is a good alternative to the standard EPR method due to its being fast, accurate, inexpensive, and simple to perform.

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.5b01478DOI Listing

Publication Analysis

Top Keywords

epr spectroscopy
16
teac values
16
antioxidant capacity
12
based mathematical
8
mathematical model
8
spectroscopy measurements
8
μmol te/100
8
proposed mathematical
8
mathematical equation
8
teac
6

Similar Publications

Natural terpenes II. Concentration-dependent profile of effects on dynamic organization of biological and model membranes.

Biochem Biophys Res Commun

December 2024

Universidad Nacional de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, Departamento de Química, Cátedra de Química Biológica, Córdoba, Argentina; CONICET, Instituto de Investigaciones Biológicas y Tecnológicas (IIByT). Córdoba, Argentina. Electronic address:

Monoterpenes (MTs), the major constituents of plant essential oils, cover a broad spectrum of biological activities through their interaction with biomembranes. MTs are highly hydrophobic substances with a net electrical dipole, but are not clearly amphipathic. As a result, they aggregate at increasing concentrations in aqueous media, and in membrane environments their behavior changes from dynamics modulators to disruptors.

View Article and Find Full Text PDF

This article investigates the influence of dopant molecules on the structural and dynamic properties of lipid bilayers in liposomes, with a focus on the effects of dopant concentration, size, and introduced electric charge. Experimental studies were performed using electron paramagnetic resonance (EPR) spectroscopy with spin probes, complemented by Monte Carlo simulations. Liposomes, formed via lecithin sonication, were doped with compounds of varying concentrations and analyzed using EPR spectroscopy to assess changes in membrane rigidity.

View Article and Find Full Text PDF

The bacterium responsible for Lyme disease, , accumulates high levels of manganese without iron and possesses a polyploid genome, characteristics suggesting potential extreme resistance to radiation. Contrary to expectations, we report that wild-type B31 cells are radiosensitive, with a gamma-radiation survival limit for 10 wild-type cells of <1 kGy. Thus, we explored radiosensitivity through electron paramagnetic resonance (EPR) spectroscopy by quantitating the fraction of Mn present as antioxidant Mn metabolite complexes (H-Mn).

View Article and Find Full Text PDF

Insights into Folding and Molecular Environment of Lyophilized Proteins Using Pulsed Electron Paramagnetic Resonance Spectroscopy.

Mol Pharm

December 2024

Pharmaceutical Technology and Biopharmaceutics, Department of Pharmacy, Ludwig-Maximilians-Universität München, Butenandtstrasse 5-13, 81377 Munich, Germany.

There is still an insufficient understanding of how the characteristics of protein drugs are maintained in the solid state of lyophilizates, including aspects such as protein distances, local environment, and structural preservation. To this end, we evaluated protein folding and the molecules' nearest environment by electron paramagnetic resonance (EPR) spectroscopy. Double electron-electron resonance (DEER) probe distances of up to approximately 200 Å and is suitable to investigate protein folding, local concentration, and aggregation, whereas electron spin echo envelope modulation (ESEEM) allows the study of the near environment within approximately 10 Å of the spin label.

View Article and Find Full Text PDF

In subsurface water, humic acid (HA) can react with active chlorine to form carcinogenic compounds, posing ecological issues and health risks. This study aims to create sludge activated carbon (SAC), combine it with Fe, and activate peroxosulfate (PMS) to remove HA from water. To verify the successful modification of SAC, the physicochemical properties were characterized using various methods such as scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), Brunauer Emmett Teller (BET) analysis, and X-ray photoelectron spectroscopy (XPS).

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!