Antibacterial Activity of Essential Oils against Uropathogens Isolated from Patients in Selected Hospitals in Bushenyi District, Uganda.

Br Microbiol Res J

Department of Biomolecular Resources and Biolab Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, P.O. Box 7062, Kampala Uganda.

Published: January 2015

Aims: To determine antibacterial activity of essential oils against bacterial uropathogens.

Study Design: A cross sectional and experimental study.

Place And Duration Of Study: Six selected hospitals in Bushenyi District, Uganda between June 2012 and July 2013.

Methodology: Clean catch midstream urine samples were collected and inoculated on Cystine Lysine Electrolyte Deficient (CLED) agar. The plates were incubated at 37°C for 24hrs to 48hrs. The essential oils were extracted by hydrodistillation of leaves for 4hrs using a Clevenger apparatus. The oil was collected and dried over anhydrous sodium sulphate (NaSO) and kept at 4°C till further use. The antimicrobial activity of essential oils against isolates was determined by agar well method. The MIC of essential oil extract was carried out by microbroth dilution method.

Results: Of the three hundred (300) midstream urine samples collected, 67(22.33%) had significant bacterial growth. is the most common isolate (61.19%, n = 41). The essential oil from showed activity against isolates of , and with mean zone of inhibition (ZI) ranging from 10-22 mm. The essential oils had no inhibitory activity on The minimum inhibitory concentration (MIC) for essential oils ranged from 0.78 to 22 μg/ml. This study showed that essential oils had MIC value of 0.78 μg/ml against and MIC values ranging from 3 to 22 μg/ml against the other tested isolates.

Conclusion: The most common uropathogen was (61.19% n = 41). essential oils exhibited antibacterial activity against majority of the uropathogens, except mean ZI of 10-22 mm and MIC of 0.78 - 22 μg/ml.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479284PMC
http://dx.doi.org/10.9734/BMRJ/2015/17526DOI Listing

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