A mathematical model of erythrocyte thermohemolysis valid also for other types of hemolysis with S-shape kinetics is proposed. A formula is derived which, when entered into the computer programme, allows to obtain the distribution of erythrocytes according to the parameter reflecting their thermostability automatically from the experimental kinetic curve of erythrocyte thermohemolysis.
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The activation energy of thermohemolysis of erythrocytes changes from 36 +/- 5 kcal/mol (35-45 degrees C) to 97 +/- 5 kcal/mol (45-55 degrees C) at the temperature about 45 degrees C in isotonic buffer. The break on Arhenius' plot is preserved also when erythrocytes are placed into plasma. The character of Arhenius' plot is the same when erythrocyte hemoglobin is totally oxidated into methemoglobin by chemical way, though thermal stability of such erythrocytes is decreased.
View Article and Find Full Text PDFA mathematical model of erythrocyte thermohemolysis valid also for other types of hemolysis with S-shape kinetics is proposed. A formula is derived which, when entered into the computer programme, allows to obtain the distribution of erythrocytes according to the parameter reflecting their thermostability automatically from the experimental kinetic curve of erythrocyte thermohemolysis.
View Article and Find Full Text PDFMeasurements were carried out of the rate constants of erythrocyte thermohemolysis and hemoglobin thermoaggregation in various animal species and also for the man under the action of hemoglobin-modifying factors (CO2, O2, ethanol). It is concluded from the obtained correlation dependences that hemoglobin thermodenaturation initiates erythrocyte thermohemolysis. The identity of the early stages of erythrocyte thermohemolysis and aging is suggested.
View Article and Find Full Text PDFThe activation energy of thermodenaturation of erythrocyte spectrin was defined by two different methods as Esp = 218 +/- 4 kcal/mole. This value was more than two times greater than that of thermohemolysis activation energy (Eth = 99 +/- 5 kcal/mole). The conclusion is made that spectrin does not play a significant role in thermohemolysis of erythrocytes.
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