AI Article Synopsis
- The study analyzed the methylome of chickens vaccinated with an Infectious laryngotracheitis (ILT) vaccine, using MBD-Seq to identify 1,155 differentially methylated regions (DMRs) in their genomes.
- Global DNA methylation levels decreased in the vaccinated birds, resulting in 704 hypomethylated and 451 hypermethylated DMRs, influencing several gene networks related to cancer and immune response.
- The research revealed a negative correlation between DNA methylation changes and the expression of seventeen out of eighteen genes studied, enhancing our understanding of ILT mechanisms.
Article Abstract
In this study we investigated the methylome of chickens immunized with Infectious laryngotracheitis (ILT) vaccine derived from chicken embryos. Methyl-CpG binding domain protein-enriched genome sequencing (MBD-Seq) method was employed in the detection of the 1,155 differentially methylated regions (DMRs) across the entire genome. After validation, we ascertained the genomic DMRs distribution and annotated them regarding genes, transcription start sites (TSS) and CpG islands. We found that global DNA methylation decreased in vaccinated birds, presenting 704 hypomethylated and 451 hypermethylated DMRs, respectively. Additionally, we performed an enrichment analysis detecting gene networks, in which cancer and RNA post-transcriptional modification appeared in the first place, followed by humoral immune response, immunological disease and inflammatory disease. The top four identified canonical pathways were EIF2 signaling, regulation of EIF4 and p70S6K signaling, axonal guidance signaling and mTOR signaling, providing new insight regarding the mechanisms of ILT etiology. Lastly, the association between DNA methylation and differentially expressed genes was examined, and detected negative correlation in seventeen of the eighteen genes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4481310 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0100476 | PLOS |
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