Simple and efficient isolation of cordycepin from culture broth of a Cordyceps militaris mutant.

J Biosci Bioeng

Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui-shi, Fukui 910-8507, Japan; Translational Research Center, Organization for Life Science Advancement Programs, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan. Electronic address:

Published: December 2015

Isolation of cordycepin from the culture broth of Cordyceps militaris mutant was investigated. Based on the solubility curve, three crystallizing processes, temperature shift (process I), pH shift (process II), and pH shift followed by temperature shift (process III) were carried out. Process III was the most promising method regarding both purity and yield.

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http://dx.doi.org/10.1016/j.jbiosc.2015.04.008DOI Listing

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