Salmon sperm DNA was used as a blocking agent to reduce background fluorescence signals from gelatin-coated cell culture dishes.
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Species-specific satellite DNA composition dictates formation of PRC1-mediated pericentric heterochromatin.
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Department of Biology, University of Pennsylvania, Philadelphia, PA, 19104.
Pericentromeres are heterochromatic regions adjacent to centromeres that ensure accurate chromosome segregation. Despite their conserved function, they are composed of rapidly evolving A/T-rich satellite DNA. This paradoxical observation is partially resolved by epigenetic mechanisms that maintain heterochromatin, independent of specific DNA sequences.
View Article and Find Full Text PDFEnhancement of Cell Adhesion on a Phosphorylcholine-Based Surface through the Interaction with DNA Mediated by Ca Ions.
J Phys Chem B
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Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-8656 Tokyo, Japan.
2-Methacryloyloxyethyl phosphorylcholine (MPC) has a PC group and is one of the most well-known bioinert polymers. In this study, we evaluated the interaction between MPC and DNA, which specifically interacts with the phospholipid head group via Ca ions. A MPC monolayer and poly(MPC) brush were fabricated to observe the effect of the structure on the interaction between MPC and DNA via Ca ions.
View Article and Find Full Text PDFDamage to Sperm DNA Mediated by Reactive Oxygen Species: Its Impact on Human Reproduction and the Health Trajectory of Offspring.
Adv Exp Med Biol
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Priority Research Centre in Reproductive Science, University of Newcastle, Callaghan, NSW, 2308, Australia.
Disruptions to the genetic integrity of the mammalian spermatozoon play a major role in determining the subsequent developmental trajectory of the embryo. This chapter examines the causative links that connect DNA damage in human spermatozoa and the appearance of mutations in the progeny responsible for a variety of clinical conditions from autism to cancer. Integral to this discussion is an abundance of evidence indicating that human spermatozoa are vulnerable to free radical attack and the generation of oxidative DNA damage.
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Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Republic of Korea.
Salmon sperm DNA was used as a blocking agent to reduce background fluorescence signals from gelatin-coated cell culture dishes.
View Article and Find Full Text PDFEnergetics of echinomycin binding to DNA.
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Department of Chemistry, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA.
Article Synopsis
- The study uses differential scanning calorimetry and UV thermal denaturation to analyze how the peptide antibiotic echinomycin interacts with DNA, providing a complete thermodynamic profile.
- The findings report a free energy change (DeltaG) of -7.6 kcal/mol, indicating the binding is favorable, with a positive enthalpy (DeltaH = +3.8 kcal/mol) and a significant positive entropy (DeltaS = +38.9 cal/mol·K) at 20°C.
- The results suggest that while the interaction is mainly driven by hydrophobic forces, direct molecular recognition, including hydrogen bonding and van der Waals interactions, also contributes significantly to the stability of the echinomycin-DNA complex.
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