AI Article Synopsis
- The text outlines protocols for using DPP1 and Factor Xa enzymes to purify proteins, ensuring that no vector or protease-related amino acids are present in the final product.
- The TAGZyme system utilizes exoprotease DPP1, which allows for the efficient recovery of proteins and integrates a His tag for easy removal through IMAC.
- Factor Xa can similarly be eliminated from the protein preparation via Xa Removal Resin, streamlining the purification process.
Article Abstract
Here, we present protocols describing the use of the dipeptidyl-aminopeptidase-1 (DPP1, DAPase) exoprotease-based TAGZyme system and the endoprotease, Factor Xa. Both enable the recovery of proteins free of any amino acids encoded by the vector and/or protease recognition site. They also provide the possibility of removing the proteases from the preparation of the target protein by a simple subtractive chromatography step. TAGZyme enzymes contain an uncleavable His tag for removal by Immobilized Metal Ion Affinity Chromatography (IMAC). Factor Xa can be removed using Xa Removal Resin.
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| http://dx.doi.org/10.1016/bs.mie.2014.11.009 | DOI Listing |
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