Introduction: The present study aimed to evaluate the antibacterial effect of the combined use of sodium hypochlorite (NaOCl) and root canal sealers on Enterococcus faecalis biofilms using a dentin infection model.
Methods: Cells of E. faecalis were introduced into the dentinal tubules by centrifugation and incubated in brain-heart infusion for 3 weeks. The biofilms in dentin were first subjected to 5% NaOCl or sterile water for 10 minutes followed by an equal thickness of AH Plus (Dentsply International Inc, York, PA), Endosequence BC Sealer (Brasseler USA, Savannah, GA), or MTA Fillapex (Angelus Indústria de Produtos Odontológicos S/A, Londrina, Brazil) placed on the root canal wall of the dentin specimens for 7, 30, and 60 days. Gutta-percha and water were used in a similar manner as controls. The proportions of dead and live bacteria inside the dentinal tubules were assessed by confocal laser scanning microscopy and viability staining.
Results: The combined use of NaOCl and sealers (30 and 60 days) killed significantly more bacteria than NaOCl or sealers alone (P < .05). NaOCl + MTA Fillapex was the most effective antibacterial combination by killing 83% bacteria in dentin tubules in 60 days. Thirty and 60 days of exposure to the sealers resulted in significantly more dead bacteria in dentin biofilms than 7-day exposures (P < .05).
Conclusions: The placement of root canal sealer after NaOCl treatment enhanced antibacterial effects against E. faecalis in the dentinal tubules. Little additional effect was obtained after 30 days of exposure to sealers.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.joen.2015.04.023 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Immunophenotype and the Odontogenic Commitment of Dental Pulp Stem Cells Co-Cultured with Macrophages Under Inflammatory Conditions Is Modulated by Complex Magnetic Fields.
Int J Mol Sci
December 2024
Department of Pharmacy, "G. d'Annunzio" University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
Dental inflammatory diseases remain a challenging clinical issue, whose causes and development are still not fully understood. During dental caries, bacteria penetrate the tooth pulp, causing pulpitis. To prevent pulp necrosis, it is crucial to promote tissue repair by recruiting immune cells, such as macrophages, able to secrete signal molecules for the pulp microenvironment and thus to recruit dental pulp stem cells (DPSCs) in the damaged site.
View Article and Find Full Text PDFComparative Analysis of Fracture Resistance of Endodontic Sealer Types and Filling Methods.
Materials (Basel)
December 2024
Department of Advanced General Dentistry, College of Dentistry, Yonsei University, Seoul 03722, Republic of Korea.
With the advent of bioceramic sealers, sealers have become a more important filling material for endodontic treatment. When a solid sealer, rather than an elastic gutta-percha, occupies a significant portion of the root canal, it is unclear whether the tooth structure will be strengthened to withstand stress or whether the increased stiffness will transmit the load directly to the entire root, potentially causing root fracture. This study compared the fracture resistance and fracture patterns of roots filled with various root canal sealers, including bioceramic sealers, and each corresponding filling technique.
View Article and Find Full Text PDFIntroduction: This systematic review and meta-analysis assess the impact of the XP-endo Shaper (XPS) on postoperative pain following root canal treatment (RCTs) and compare its efficacy with other endodontic systems.
Methods: A comprehensive literature search was conducted in MEDLINE, Web of Science, Embase, and the Cochrane Library from January 2000 to August 2024. Randomized controlled trials using XPS and reporting postoperative pain were included.
Effects of nanosecond‑ and microsecond‑pulse Er, Cr: YSGG laser on the morphology and pulp temperature of dentin in dental restoration debonding.
Lasers Med Sci
January 2025
Department of Stomatology, the First Medical Centre, Chinese PLA General Hospital, Beijing, 100853, China.
Objective: This study evaluated dentin morphology and pulp cavity temperature changes during nanosecond‑ and microsecond‑pulse Er, Cr: YSGG laser debonding restoration and residual adhesive.
Materials And Methods: Ten caries-free teeth had their enamel removed perpendicular to the long axis, followed by bonding of glass ceramic restorations. The samples were randomly divided into two groups and subjected to Er, Cr: YSGG laser (3 mJ, 100 Hz, 100 ns), (3 mJ, 100 Hz, 150 µs) for debonding of restoration and residual adhesive on dentin surfaces.
Multiscale Finite Element Modeling of Human Ear for Acoustic Wave Transmission into Cochlea and Hair Cells Fatigue Failure.
J Biomech Eng
January 2025
School of Aerospace and Mechanical Engineering, University of Oklahoma, 865 Asp Ave, Norman, OK 73019, USA.
Hearing loss is highly related to acoustic injuries and mechanical damage of ear tissues. The mechanical responses of ear tissues are difficult to measure experimentally, especially cochlear hair cells within the organ of Corti (OC) at microscale. Finite element (FE) modeling has become an important tool for simulating acoustic wave transmission and studying cochlear mechanics.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!