AI Article Synopsis
- Oligonucleotide microarrays are a key method for gene diagnostics, and atomic force microscopy (AFM) enhances their analysis by providing detailed 3D images of the molecules.
- AFM's high resolution helps lower detection limits for target DNA and improve the signal-to-noise ratio, making it more effective for measuring nucleic acids on microarrays.
- The study proposes a new way to evaluate hybridization results of gold nanoparticle-labeled nucleic acids on silicon microarrays, using AFM to quantify the area occupied by the nanoparticles on the surface.
Article Abstract
Oligonucleotide microarrays are considered today to be one of the most efficient methods of gene diagnostics. The capability of atomic force microscopy (AFM) to characterize the three-dimensional morphology of single molecules on a surface allows one to use it as an effective tool for the 3D analysis of a microarray for the detection of nucleic acids. The high resolution of AFM offers ways to decrease the detection threshold of target DNA and increase the signal-to-noise ratio. In this work, we suggest an approach to the evaluation of the results of hybridization of gold nanoparticle-labeled nucleic acids on silicon microarrays based on an AFM analysis of the surface both in air and in liquid which takes into account of their three-dimensional structure. We suggest a quantitative measure of the hybridization results which is based on the fraction of the surface area occupied by the nanoparticles.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463420 | PMC |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!