Objective: This study is to establish the fingerprint and find out the common chromatographic peaks of Inula cappa by HPLC.
Method: The HPLC analysis was performed on an Agilent Eclipse Plus C18 column (2.1 mm x 150 mm, 1.8 μm) with 0.1% fomic acid aqueous solution-0.1% fomic acid acetonitrile solution as mobile phase at a flow rate of 0.3 · mL(-1) · min(-1); The detective wavelength is 325 nm; The column temperature is 45 °C.
Result: The results indicated that 5 of 17 common peaks were identified . The similarity about 10 groups of Inulacappais is over 0.95.
Conclusion: This method is able to be a scientific basis of quality assessment according to its convenient and reliable.
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Wei Sheng Yan Jiu
May 2022
Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.
Objective: To establish a rapid, accurate and sensitive method by liquid chromatography-tandem mass spectrometry with isotope internal standard dilution technique for the determination of chlorpromazine and promethazine and their metabolites in swine tissues.
Methods: The swine tissues sample was extracted with acetonitrile and purified on MCX cartridge. The liquid chromatography separation was performed on an ACQUITY UFLC® HSS T3(100 mm×2.
Objective: This study is to establish the fingerprint and find out the common chromatographic peaks of Inula cappa by HPLC.
Method: The HPLC analysis was performed on an Agilent Eclipse Plus C18 column (2.1 mm x 150 mm, 1.
J Pharm Biomed Anal
January 2014
Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH, United States.
Pomalidomide was recently approved by the United States Food and Drug Administration for the treatment of patients with relapsed or refractory multiple myeloma who have received at least two prior therapies. As pomalidomide is increasingly evaluated in other diseases and animal disease models, this paper presents development and validation of a sensitive liquid chromatography tandem mass spectrometry assay for quantification of pomalidomide in mouse plasma and brain tissue to fill a gap in published preclinical pharmacokinetic and analytical data with this agent. After acetonitrile protein precipitation, pomalidomide and internal standard, hesperitin, were separated with reverse phase chromatography on a C-18 column with a gradient mobile phase of water and acetonitrile with 0.
View Article and Find Full Text PDFSe Pu
May 2000
Zhaotong Institute for Drug Control, Zhaotong 657000, China.
An HPLC method for the quantitative determination of chlorogenic acid in Eucommia leaf was investigated. The sample was extracted with methanol solution containing 5% fomic acid and then analyzed by HPLC. The operating conditions were Shim-pack CLC-ODS column (6.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!