Human noroviruses (NoV) are the leading cause of acute viral gastroenteritis worldwide. Significant antigenic diversity of NoV strains has limited the availability of broadly reactive ligands for design of detection assays. The purpose of this work was to produce and characterize single stranded (ss)DNA aptamers with binding specificity to human NoV using an easily produced NoV target-the P domain protein. Aptamer selection was done using SELEX (Systematic Evolution of Ligands by EXponential enrichment) directed against an Escherichia coli-expressed and purified epidemic NoV GII.4 strain P domain. Two of six unique aptamers (designated M1 and M6-2) were chosen for characterization. Inclusivity testing using an enzyme-linked aptamer sorbent assay (ELASA) against a panel of 14 virus-like particles (VLPs) showed these aptamers had broad reactivity and exhibited strong binding to GI.7, GII.2, two GII.4 strains, and GII.7 VLPs. Aptamer M6-2 exhibited at least low to moderate binding to all VLPs tested. Aptamers significantly (p<0.05) bound virus in partially purified GII.4 New Orleans outbreak stool specimens as demonstrated by ELASA and aptamer magnetic capture (AMC) followed by RT-qPCR. This is the first demonstration of human NoV P domain protein as a functional target for the selection of nucleic acid aptamers that specifically bind and broadly recognize diverse human NoV strains.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jbiotec.2015.06.389 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prevalence of Human Norovirus GII.4 Sydney 2012 [P31] between 2019 and 2021 among Young Children from Rural Communities in South Africa.
Viruses
August 2023
Department of Biochemistry and Microbiology, Faculty of Science, Engineering and Agriculture, University of Venda, Private Bag X5050, Thohoyandou 0950, South Africa.
Acute gastroenteritis (AGE) accounts for considerable morbidity and mortality in the paediatric population worldwide, especially in low-income countries. Human norovirus (HNoV), particularly GII.4 strains, are important agents of AGE.
View Article and Find Full Text PDFMolecular epidemiology and temporal evolution of norovirus associated with acute gastroenteritis in Amazonas state, Brazil.
BMC Infect Dis
April 2018
Virology Section, Evandro Chagas Institute, Brazilian Ministry of Health, Rodovia BR-316, Km 7 s/n, Levilândia, Ananindeua, Pará, 67030-000, Brazil.
Background: Globally, Norovirus (NoV) is considered the most common cause of diarrheal episodes across all age groups. Despite its wide genetic diversity, the GII.4 strain is the most predominant and has been associated with epidemics worldwide.
View Article and Find Full Text PDFEarly Detection of Epidemic GII-4 Norovirus Strains in UK and Malawi: Role of Surveillance of Sporadic Acute Gastroenteritis in Anticipating Global Epidemics.
PLoS One
February 2017
Department of Clinical Infection, Microbiology & Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom.
Noroviruses are endemic in the human population, and are recognised as a leading cause of acute gastroenteritis worldwide. Although they are a highly diverse group of viruses, genogroup-II genotype-4 (GII-4) noroviruses are the most frequently identified strains worldwide. The predominance of GII-4 norovirus strains is driven by the periodic emergence of antigenic variants capable of evading herd protection.
View Article and Find Full Text PDFMolecular epidemiology of norovirus GII.4 variants in children under 5 years with sporadic acute gastroenteritis in South Korea during 2006-2013.
J Clin Virol
November 2014
Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Electronic address:
Background: The global emergence of norovirus (NoV) GII.4 variants has raised public concerns in the world including South Korea since 1996.
Objective: We analyzed seasonality and genotypic pattern for sporadic cases by norovirus GII-4 variants.
High serum levels of norovirus genotype-specific blocking antibodies correlate with protection from infection in children.
J Infect Dis
December 2014
Vaccine Research Center, University of Tampere Medical School.
Article Synopsis
- Norovirus is a leading cause of acute gastroenteritis in children, and the study focused on understanding how IgG antibodies may protect against it.
- Researchers examined serum samples from children infected with various norovirus strains to measure specific IgG levels and their blocking activity against these viruses.
- Findings showed that children infected with a specific variant (GII-4 NO) had low antibody levels before infection but developed a stronger antibody response afterward, highlighting the protection is strain-specific.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!