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Computational Design of the β-Sheet Surface of a Red Fluorescent Protein Allows Control of Protein Oligomerization. | LitMetric

Computational Design of the β-Sheet Surface of a Red Fluorescent Protein Allows Control of Protein Oligomerization.

PLoS One

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, United States of America; Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, United States of America.

Published: May 2016

AI Article Synopsis

Article Abstract

Computational design has been used with mixed success for the design of protein surfaces, with directed evolution heretofore providing better practical solutions than explicit design. Directed evolution, however, requires a tractable high-throughput screen because the random nature of mutation does not enrich for desired traits. Here we demonstrate the successful design of the β-sheet surface of a red fluorescent protein (RFP), enabling control over its oligomerization. To isolate the problem of surface design, we created a hybrid RFP from DsRed and mCherry with a stabilized protein core that allows for monomerization without loss of fluorescence. We designed an explicit library for which 93 of 96 (97%) of the protein variants are soluble, stably fluorescent, and monomeric. RFPs are heavily used in biology, but are natively tetrameric, and creating RFP monomers has proven extremely difficult. We show that surface design and core engineering are separate problems in RFP development and that the next generation of RFP markers will depend on improved methods for core design.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4468108PMC
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0130582PLOS

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