AI Article Synopsis
- - This study investigates how adipose tissue-derived cells (ADCs) affect fracture healing by using advanced imaging techniques on mice with femur defects.
- - Researchers transplanted luciferase-expressing ADCs into one femur and a control substance in the other, monitoring changes over 20 days via micro-CT and bioluminescence imaging.
- - Findings indicate that ADCs promote increased bone density around the implant site, suggesting they play a positive role in the fracture healing process despite limited cell migration.
Article Abstract
Purpose: Although the applications of adipose tissue-derived cells (ADCs) in regenerative medicine have been investigated, the role of ADCs in fracture healing remains unclear. In this study, we examined the fracture-healing effects and survival of transplanted ADCs using micro-computed tomography (CT) and bioluminescence imaging (BLI).
Materials And Methods: Luciferase-expressing ADCs were suspended in solubilized basement membrane preparation (SBMP) and xenografted on defects in the right femur of nude mice (n=5). SBMP alone was grafted on a defect in the contralateral femur. Serial in vivo micro-CT and BLI were performed for 20 days. Ex vivo BLI images of both femurs were obtained. Differences in the Hounsfield unit (HU), HU(ratio), and luciferase activities were compared using Wilcoxon signed-rank tests and non-parametric longitudinal analyses (p<0.05).
Results: In vivo BLI revealed a signal drop on day 2, reconstitution on day 5, and continuous decrement thereafter. Ex vivo BLI revealed residual activity in the ADC-implanted and adjacent areas. No activity was detected in the contralateral femur. The overall increment rate of normalized HUs was higher for ADC-treated femurs than for SBMP-treated femurs. Cell migration to distant injury sites was not detected.
Conclusion: Enhanced bone density in the implant area suggests that ADCs have fracture-healing effects.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479841 | PMC |
| http://dx.doi.org/10.3349/ymj.2015.56.4.1106 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Developing a Risk Score for Predicting Multiple Revision Surgeries in Patients With Fracture-Related Infections.
J Am Acad Orthop Surg
January 2025
From the Department of Orthopaedic Surgery, University of Alabama at Birmingham, Birmingham, AL (Yeager, Rutz, Strother, Spitler, and Johnson), and the Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL (Gross, Benson, and Carter).
Introduction: Postoperative infections are a leading cause of morbidity following fracture repair. The purpose of this study is to develop a risk score predicting fracture-related infection (FRI) that will require one versus multiple revision surgeries related to infection eradication and bone healing.
Methods: This is a retrospective cohort study conducted at a single level I trauma center from 2013 to 2020.
Restoring bone healing potential.
Elife
January 2025
Centre for Oral Immunobiology and Regenerative Medicine, Institute of Dentistry, Queen Mary University of London, London, United Kingdom.
A combination of intermittent fasting and administering Wnt3a proteins to a bone injury can rejuvenate bone repair in aged mice.
View Article and Find Full Text PDFA Rare Case Report: Managing PCL Avulsion Fracture with a Fracture of the Proximal Tibial Shaft.
J Orthop Case Rep
January 2025
Department of Orthopaedics, SN Medical College, Agra, Uttar Pradesh, India.
Introduction: Posterior cruciate ligament (PCL) avulsion fractures of the tibia with ipsilateral tibial shaft represent a rare but challenging orthopedic injury, necessitating careful consideration of surgical interventions for optimal outcomes. This case report presents the successful management of tibial shaft fracture (proximal 1/3rd junction) along with ipsilateral PCL avulsion fracture of tibia using a novel approach using open reduction internal fixation (ORIF) of tibial shaft with Locking compression plate (LCP) with cannulated cancellous screw and spiked washer fixation of PCL avulsion. There are no case reports or research articles available for the management of PCL avulsion fracture of tibia associated with ipsilateral tibial shaft fracture.
View Article and Find Full Text PDFTreatment of a collapse fracture of the anterolateral tibial plateau with a lateral locking plate and the Jail screw technique.
BMC Surg
January 2025
Department of Orthopedics, Nanjing Drum Tower Hospital Group Suqian Hospital, Suqian, China.
Background: To explore the advantages of a lateral tibial locking plate combined with Jail screw fixation in the treatment of anterolateral tibial plateau collapse fracture (ATPCF).
Methods: A retrospective analysis was conducted on patients with ATPCFs admitted to our hospital from February 2019 to February 2023. Twenty-six patients were successfully included, including 15 males and 11 females, with an average age of 58.
Low-grade infections in nonunion of the femur and tibia without clinical suspicion of infection - Incidence, microbiology, treatment, and outcome.
Injury
January 2025
Institute for Biomechanics, Paracelsus Medical University, Strubergasse 21, 5020 Salzburg, Austria; Department of Trauma Surgery, BG Unfallklinik Murnau, Professor-Küntscher-Str. 8, 82418 Murnau am Staffelsee, Germany.
Treatment algorithms for fracture nonunion depend on the presence or absence of bacterial infection. However, it is often impossible to identify infection preoperatively. While some infections may present with clinical signs of infection, low-grade infections lack infection signs and have a clinical presentation similar to aseptic nonunion.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!