Proteolysis in model Portuguese cheeses: Effects of rennet and starter culture.

Food Chem

Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, P-4200-072 Porto, Portugal. Electronic address:

Published: June 2008

AI Article Synopsis

  • The study investigates how proteolysis (the breakdown of proteins) occurs in traditional Portuguese cheeses made from bovine milk, using either animal or plant rennet.
  • It examines the impact of two strains of lactic acid bacteria, Lactococcus lactis and Lactobacillus brevis, on protein breakdown, both alone and in combination.
  • Results show that while rennet affects the extent of proteolysis, it does not influence its depth, and the bacteria strains help increase the release of smaller peptides and free amino acids in cheese.

Article Abstract

To shed further light onto the mechanisms of proteolysis that prevail throughout ripening of Portuguese cheeses, model cheeses were manufactured from bovine milk, following as much as possible traditional manufacture practices - using either animal or plant rennet. The individual role upon proteolysis of two (wild) strains of lactic acid bacteria - viz. Lactococcus lactis and Lactobacillus brevis, which are normally found to high viable numbers in said cheeses, was also considered, either as single or mixed cultures. Our experimental results confirmed the influence of rennet on the proteolysis extent, but not on proteolysis depth. On the other hand, the aforementioned strains clearly improved release of medium- and small-sized peptides, and contributed as well to the free amino acid pool in cheese.

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Source
http://dx.doi.org/10.1016/j.foodchem.2007.11.050DOI Listing

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