We find yeast cell surface display can be used to engineer enzymes by selecting the enzyme library for high affinity binding to reaction intermediates. Here we cover key steps of enzyme engineering on the yeast cell surface including library design, construction, and selection based on magnetic and fluorescence-activated cell sorting.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648535 | PMC |
| http://dx.doi.org/10.1007/978-1-4939-2748-7_14 | DOI Listing |
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