The study of immune cell migration is important for understanding the immune system network, which is associated with the response to foreign cells. Neutrophils act against foreign cells before any other immune cell, and they must be able to change shape and squeeze through narrow spaces in the extracellular matrix (ECM) during migration to sites of infection. Conventional in vitro migration assays are typically performed on two-dimensional substrates that fail to reproduce the three-dimensional (3-D) nature of the ECM. Here we present an in vitro method to simulate the 3-D migration of neutrophils using an electrospun nanofibrous membrane, which is similar to the ECM in terms of morphology. We examined the properties of neutrophil movement and the effects of gravity and the presence of IL-8, which has been widely used as a chemotactic attractant for neutrophils. The number of neutrophils passing through the nanofibrous membrane were higher, and their movement was more active in the presence of IL-8. Also, we confirmed that neutrophils could migrate against gravity toward IL-8 through a nanofibrous membrane.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2144/000114297 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Quaternized Nanofiber-Based Anion-Exchange Chromatography Membrane with Periodic Diagonal Surface Structure for Efficient Protein Separation.
ACS Appl Mater Interfaces
January 2025
Key Laboratory of Textile Fiber and Products, Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Intelligent Textile Materials & Application, Wuhan Textile University, Wuhan 430200, China.
Constructing a nanofibrous membrane with high flow rate surface pore structure and high-density ligand chemical structure is a promising strategy to balance the trade-off between high flow rates and high adsorption capacity for protein separation and purification. Herein, a nanofiber-based ion-exchange chromatography membrane with a periodic diagonal surface structure and high ionic strength ligands was fabricated using dispersion cross-linking, wet coating, and template printing with a three-wire diagonal woven mesh. For this membrane, EVOH nanofibers were used as skeleton, glutaraldehyde (GA) as cross-linking agent, and quaternized chitosan (QCS) as binder and functional ligand.
View Article and Find Full Text PDFEngineered Lubricative Lecithin-Based Electrospun Nanofibers for the Prevention of Postoperative Abdominal Adhesion.
Pharmaceutics
December 2024
Basic Research Key Laboratory of General Surgery for Digital Medicine, Affiliated Hospital of Hebei University, Baoding 071000, China.
: Postoperative abdominal adhesion is a prevalent complication following abdominal surgery, with the incidence of adhesion reaching up to 90%, which may precipitate a range of adverse outcomes. Although fibrous membranes loaded with various anti-inflammatory or other drugs have been proposed for anti-adhesion, most of them suffer from drug-induced adverse effects. : In this study, a lecithin-based electrospun polylactic acid (PLA) nanofibrous membrane (L/P-NM) was developed for the prevention of postoperative abdominal adhesion, utilizing the hydration lubrication theory.
View Article and Find Full Text PDFEffect of Promising Sustainable Nano-Reinforcements on Polysulfone/Polyvinylpyrrolidone-Based Membranes: Enhancing Mechanical Properties and Water Filtration Performance.
Polymers (Basel)
December 2024
Department of Environmental Engineering, Istanbul University-Cerrahpaşa, Avcilar, Istanbul 34320, Turkey.
In this study, polysulfone/polyvinylpyrrolidone (PSf/PVP, 20 wt%/5 wt%)-based ultrafiltration (UF) membranes reinforced with different ratios (0.5 and 1 wt%) of cellulose nanocrystals (CNCs) and cellulose nanofibres (CNFs) were prepared by the phase inversion method. The effect of CNC, CNF, and CNC-CNF reinforcement on the morphology, roughness, crystallinity, porosity, average pore size, mechanical properties, and filtration performance of PSf/PVP-based membrane was investigated.
View Article and Find Full Text PDFDevelopment of Betalain-immobilized polylactic acid nanofibers as a green and sustainable sensor for toxic ammonia.
Int J Biol Macromol
December 2024
National Research Centre (Scopus Affiliation ID 60014618), Textile Industries Research Division, Pre-treatment and Finishing of Cellulose Based Textiles Department, 33 El-Buhouth St., (former El-Tahrir St.), Dokki, P.O. 12622, Giza, Egypt.
Ammonia has been an important industrial colorless agent. Exposure to gaseous ammonia results in organ damage or even death. Herein, an environmentally friendly colorimetric detector for aqueous and gaseous ammonia was prepared utilizing vapochromic polylactic acid nanofibers.
View Article and Find Full Text PDFSterilization and Filter Performance of Nano- and Microfibrous Facemask Filters - Electrospinning and Restoration of Charges for Competitive Sustainable Alternatives.
Macromol Rapid Commun
December 2024
Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomimetic Membranes and Textiles, St. Gallen, 9014, Switzerland.
Facemask materials have been under constant development to optimize filtration performance, wear comfort, and general resilience to chemical and mechanical stress. While single-use polypropylene meltblown membranes are the established go-to material for high-performing mask filters, they are neither sustainable nor particularly resistant to sterilization methods. Herein an in-depth analysis is provided of the sterilization efficiency, filtration efficiency, and breathing resistance of selected aerosol filters commonly implemented in facemasks, with a particular focus on the benefits of nanofibrous filters.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!