AI Article Synopsis
- Researchers developed a new larger base pair involving adenine (A) and a nucleobase analogue called 4-hydroxy-2-mercaptobenzimidazole (SB).
- The study focused on the incorporation of a 2'-deoxynucleoside-5'-triphosphate with the SB analogue (dSBTP) into DNA sequences using different DNA polymerases.
- The successful integration of dSBTP opposite dA in DNA templates opens new opportunities for creating non-natural base pairs in biotechnology.
Article Abstract
Here, we describe the enzymatic construction of a new larger base pair formed between adenine (A) and a 4-hydroxy-2-mercaptobenzimidazole (SB) nucleobase analogue. We investigated the enzymatic incorporation of 2'-deoxynucleoside-5'-triphosphate bearing a SB nucleobase analogue (dSBTP) into oligonucleotides (ONs) by DNA polymerases. dSBTP could be effectively incorporated at the site opposite a dA in a DNA template by several B family DNA polymerases. These findings provide new insights into various aspects of biotechnology, including the design of non-natural base pairs.
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| http://dx.doi.org/10.1016/j.bmcl.2015.05.075 | DOI Listing |
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