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Western blotting using microfluidics. | LitMetric

Western blotting using microfluidics.

Methods Mol Biol

Division of Cardiovascular Sciences, National Heart, Lung and Blood Institute, National Institutes of Health, 6701 Rockledge Drive, Room 10214, MSC 7936, Bethesda, MD, 20892-7956, USA,

Published: February 2016

Together with polyacrylamide gel electrophoresis, the western blot has been an invaluable research technique in biological sciences. It continues to serve as an important diagnostic tool in medical laboratories. The procedure, however, involves multiple steps that are often time and resource intensive in addition to being of low throughput. Using advances in microfluidics, Hughes and Herr et al. initially developed a microfluidic western blot approach that significantly optimizes resources and assay times. More recent developments have enabled multiplexing to facilitate probing of multiple proteins.

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http://dx.doi.org/10.1007/978-1-4939-2694-7_48DOI Listing

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