Western blotting enables the detection and characterization of proteins of low abundance. Sodium dodecyl sulfate (SDS) polyacrylamide gel-separated proteins are normally transferred electrophoretically to nitrocellulose or polyvinylidene difluoride membranes. Here we describe the transfer proteins [Ro 60 (or SSA) autoantigen, 220 and 240 kDa spectrin antigens, and prestained molecular weight standards] by diffusion from SDS polyacrylamide gels at 37 °C. Up to 12 immunoblots can be obtained from a single gel by this method.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305815 | PMC |
| http://dx.doi.org/10.1007/978-1-4939-2694-7_11 | DOI Listing |
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