AI Article Synopsis
- Effective Arachidonic Acid (ARA) production using Mortierella alpina was optimized in a 30-L airlift bioreactor by adjusting aeration rates and temperatures.
- Significant variations in aeration rates (0.6-1.8 vvm) and dynamic temperature control (13-28°C) improved cell growth and ARA yield.
- The combined strategy resulted in an ARA production peak of 5.8 g/L, marking a notable improvement over traditional methods.
Article Abstract
Effective production of arachidonic acid (ARA) using Mortierella alpina was conducted in a 30-L airlift bioreactor. Varying the aeration rate and temperature significantly influenced cell morphology, cell growth, and ARA production, while the optimal aeration rate and temperature for cell growth and product formation were quite different. As a result, a two-stage aeration rate control strategy was constructed based on monitoring of cell morphology and ARA production under various aeration rate control levels (0.6-1.8 vvm). Using this strategy, ARA yield reached 4.7 g/L, an increase of 38.2% compared with the control (constant aeration rate control at 1.0 vvm). Dynamic temperature-control strategy was implemented based on the fermentation performance at various temperatures (13-28°C), with ARA level in total cellular lipid increased by 37.1% comparing to a constant-temperature control (25°C). On that basis, the combinatorial fermentation strategy of two-stage aeration rate control and dynamic temperature control was applied and ARA production achieved the highest level of 5.8 g/L.
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| http://dx.doi.org/10.1080/10826068.2015.1031397 | DOI Listing |
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