AI Article Synopsis
- This study investigates how cobalt nanoparticles affect osteoclasts, focusing on their toxicity and biological activity in relation to bone loss (osteolysis).
- Methods included culturing RAW 264.7 monocyte-macrophages and inducing them to become osteoclast-like cells, followed by testing the toxicity of cobalt nanoparticles and cobalt chloride at various concentrations and times using the MTT test and Q-PCR to measure mRNA expression.
- Findings revealed that cobalt nanoparticles inhibit osteoclast proliferation at most concentrations, but surprisingly improve the expression of specific markers (CA II and Cat K) at 10-50 μM concentrations, highlighting their complex biological effects.
Article Abstract
Objective: To explore the toxicity and biological activity of cobalt nanoparticles on the osteoclasts. Analyze the relationship between cobalt nanoparticles and osteolysis.
Methods: Monocyte-macrophages (RAW 264.7) was cultured in vitro, osteoclast-like cells were induced by lipopolysaccharides (LPS). After RAW 264.7 was induced for 24 h, Methyl Thiazolium Tetrazolium (MTT) biological toxicity test of osteoclast-like cell was preceded using Cobalt nanoparticles (set 4 concentrations: 10, 20, 50, 100 μM) and cobalt chloride (set 4 concentrations: 10, 20, 50, 100 μM) at 2, 4, 8, 24 and 48 h respectively. The relative expression of mRNA of CA II and Cat K after RAW 264.7 induction was determined by Q-PCR.
Results: mRNA relative expression of CA II, Cat K were reduced at multiple concentrations both cobalt nanoparticles and cobalt chloride, and was time and concentration dependent, cobalt nanoparticles are more significant than cobalt chloride group. But when the cobalt nanoparticles concentration is in 10-50 μM, the mRNA relative expression of CA II, Cat K increased.
Conclusion: Cobalt nanoparticles have biological toxicity. At multiple concentrations, the differentiation and proliferation of osteoclasts was inhibited, but when the concentration of cobalt nanoparticles is in 10-50 μM, it has been strengthened.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6583723 | PMC |
| http://dx.doi.org/10.1111/os.12180 | DOI Listing |
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