AI Article Synopsis
- Affinity chromatography requires specific ligands for effective protein separations, but antibodies may not always be the best option due to technical and economic limitations.
- To find suitable ligands, researchers can use pre-established combinatorial libraries, which provide a broad range of structural options.
- This review highlights various common affinity ligand libraries and the best screening methods to identify the right ligands for different types of libraries, including a comparative analysis.
Article Abstract
Affinity chromatography separations of proteins call for highly specific ligands. Antibodies are the most obvious approach; however, except for specific situations, technical and economic reasons are arguments against this choice especially for preparative purposes. With this in mind, the rationale is to select the most appropriate ligands from collections of pre-established molecules. To reach the objective of having a large structural coverage, combinatorial libraries have been proposed. These are classified according to their nature and origin. This review presents and discusses the most common affinity ligand libraries along with the most appropriate screening methods for the identification of the right affinity chromatography selective structure according to the type of library; a side-by-side comparison is also presented.
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| http://dx.doi.org/10.1002/jssc.201500285 | DOI Listing |
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