The evaluation of content of DNA of lactobaccilli and particular types of aerobic anaerobic opportunistic bacteria in sampling of scrapes from urogenital tract offemale patients of the network laboratory INVITRO was implemented. The technique of polymerase chain reaction in real-time was implemented. It is demonstrated that decreasing of content of lactobaccilli in total bacterial mass isfollowed by increasing of occurrence, concentration and relative content of all types of opportunistic pathogens except ureaplasmna. These changes are expressed in different degree for different types of opportunistic pathogens. The increasing of varieties of types of microflora of urogenital tract under decreasing of content of lactobaccilli is noted.
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PLoS One
January 2025
Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, United States of America.
Urinary tract infections (UTIs) are among the most common bacterial infections of both dogs and humans, with most caused by uropathogenic Escherichia coli (UPEC). Recurrent UPEC infections are a major concern in the treatment and management of UTIs in both species. In humans, the ability of UPECs to form intracellular bacterial communities (IBCs) within urothelial cells has been implicated in recurrent UTIs.
View Article and Find Full Text PDFPLoS One
January 2025
Department of Kidney Transplantation, Nephropathy Hospital, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaan'xi, China.
Increasing evidence suggests that dysbiosis of gut microbiota exacerbates chronic kidney disease (CKD) progression. Curcumin (CUR) has been reported to alleviate renal fibrosis in animal models of CKD. However, the relationship between CUR and gut microbiome in CKD remains unclear.
View Article and Find Full Text PDFReprod Fertil Dev
January 2025
Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Context The adipose tissue produces adipokines - hormones essential to many biological functions, including reproduction. Aims We hypothesised that resistin, one of the adipokines, is present in the blood plasma, uterine luminal flushings (ULF) and uterus of pigs during the oestrous cycle and early pregnancy, and that resistin influences uterine steroidogenesis. Methods This study aimed to determine the expression of resistin in the porcine endometrium and myometrium during the cycle and pregnancy by quantitative real-time polymerase chain reaction and western blot (WB).
View Article and Find Full Text PDFStem cells exist within a niche, a microenvironment that regulates their activity, but the mechanisms by which niche cells influence stem cell behaviour are poorly understood. In this issue, Stephen DiNardo and colleagues reveal that the shape of the adult Drosophila testes niche, which is dependent on the cytoskeleton of the niche cells, is crucial to maintaining germinal stem cell function. To learn more about this work, we spoke to first author Gabriela Vida and corresponding author Stephen DiNardo, Professor of Cell and Developmental Biology at the University of Pennsylvania, USA.
View Article and Find Full Text PDFBackground: Renal atrophy may reflect an end organ consequence of chronic vascular disease. Renal volume loss may therefore provide a window into brain aging and Alzheimer disease risk.
Method: We obtained whole-body 1.
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