Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488387 | PMC |
| http://dx.doi.org/10.1038/nmeth.3398 | DOI Listing |
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