Lipid/DNA co-extraction from one sample is attractive in limiting biases associated with microbial community analysis from separate extractions. We sought to enhance established co-extraction methods and use high-throughput 16S rRNA sequencing to identify preferentially extracted taxa from co-extracted DNA. Co-extraction results in low DNA yields and distinct community structure changes.
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http://dx.doi.org/10.1016/j.mimet.2015.05.018 | DOI Listing |
Anal Chim Acta
February 2025
Department of Chemistry, Iowa State University, Ames, IA, 50011, USA. Electronic address:
Background: Infections from the hepatitis B virus (HBV) are a major risk factor for hepatocellular carcinoma, one of the most common types of liver cancer. Circulating cell-free DNA (ccfDNA) in human plasma can be used as a non-invasive biomarker for diagnosing HBV-related liver diseases. The isolation of target ccfDNA sequences is often challenging due to the co-extraction of highly abundant non-target DNA from samples.
View Article and Find Full Text PDFJ Hazard Mater
January 2025
Shenzhen Research Institute of Xiamen University, Shenzhen 518000, China; College of the Environment and Ecology, Xiamen University, Xiamen 361005, China; Fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies, Xiamen 361005, China. Electronic address:
Due to the distinct difference in chemical properties, analysis of organic pollutants and heavy metals generally employs different sample preparation and measurement techniques, resulting in low analytical efficiency and high cost. To this end, a strategy for the co-extraction and then simultaneous quantification of organic pollutants and heavy metals was proposed by the on-line hyphenation of magnetic field-assisted in-tube solid phase microextraction (MA/IT-SPME) and HPLC technique. Simultaneous analysis of triazoles and chromium species were adopted as paradigm to demonstrate the feasibility of the proposed strategy.
View Article and Find Full Text PDFRSC Adv
January 2025
Department of Chemistry, Institute of Technical Education and Research, Siksha 'O' Anusandhan Deemed to Be University Bhubaneswar-751030 Odisha India
The widespread use of neodymium-iron-boron (NdFeB) magnets has raised concerns about the environmental impact of their disposal, prompting the need for sustainable recycling strategies. Traditional solvents used in recycling are toxic and flammable, making them risky to use. Ionic liquids are safer and greener options with low vapor pressure, high stability, and less flammability.
View Article and Find Full Text PDFRSC Adv
January 2025
Waste Recycling Technologies, Materials & Chemistry Unit, Flemish Institute for Technological Research, VITO N.V. Boeretang 200 B-2400 Mol Belgium
Antimony is a critical raw material in Europe wherein for 43% of its market share it is applied in the form of antimony trioxide as a fire retardant in plastics. Currently, antimony recycling from waste plastics does not take place and has been scarcely studied. In this work, a process was developed to extract antimony from a soft PVC material and recover it as SbClO.
View Article and Find Full Text PDFDalton Trans
January 2025
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China.
The co-extraction of pertechnetate (TcO) along with the uranyl ion (UO) is a problematic issue that complicates the Plutonium Uranium Reduction EXtraction (PUREX) process for reprocessing spent nuclear fuel. Unfortunately, research on the interactions of TcO with UO is very scarce. This work quantitatively investigated the interactions of TcO and its analog ReO with UO in acetonitrile with 0.
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