Co-extraction of DNA and PLFA from soil samples.

J Microbiol Methods

Department of Microbiology, College of Arts & Sciences, University of Tennessee, Knoxville, TN 37996, United States; Department of Civil & Environmental Engineering, College of Engineering, University of Tennessee, United States; Department of Earth & Planetary Sciences, College of Arts and Sciences, University of Tennessee, United States; Center for Environmental Biotechnology, University of Tennessee, United States. Electronic address:

Published: August 2015

Lipid/DNA co-extraction from one sample is attractive in limiting biases associated with microbial community analysis from separate extractions. We sought to enhance established co-extraction methods and use high-throughput 16S rRNA sequencing to identify preferentially extracted taxa from co-extracted DNA. Co-extraction results in low DNA yields and distinct community structure changes.

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http://dx.doi.org/10.1016/j.mimet.2015.05.018DOI Listing

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