[HPLC fingerprint analysis of flavonoids of phyllanthi fructus from different habitats].

Objective: To establish the HPLC fingerprint of flavonoids of Phyllanthi Fructus from different habitats.

Methods: HPLC method was adopted. The flavonoids composition of Phyllanthi Fructus from 10 different habitats was determined on an Agilent C, chromatographic column with 0. 5% formic acid water (A)-acetonitrile (B) as the mobile phase in gradient elution under the wavelength of 254 nm. The HPLC fingerprints of flavonoids composition of Phyllanthi Fructus were established to evaluate the qualitiy of them.

Results: The HPLC fingerprints of flavonoids composition of Phyllanthi Fructus from 10 different habitats were established. 18 common peaks were found and the similarities of them were more than 0. 90 except the ones from Guangxi and Guangdong.

Conclusion: The method is simple, accurate and repeatable. It can be used for research and quality control of the effective components in Phyllanthi Fructus.