A method has been developed to quantify the nitrofuran metabolites 3-amino-5-morphorinomethyl-1,3-oxazolidinone, 3-amino-oxazolidinone, 1-aminohydantoin, and semicarbazide, as well as chloramphenicol in shrimp with a single extraction procedure followed by LC-MS/MS analysis. Dynamic selected reaction monitoring with positive and negative ionization mode switching was used. The method LODs were 0.5 ng/g for the nitrofuran metabolites and 0.3 ng/g for chloramphenicol.
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http://dx.doi.org/10.5740/jaoacint.14-261 | DOI Listing |
Anal Bioanal Chem
December 2024
Chemistry Institute (IQ), Laboratory for the Support of Technological Development (Ladetec), Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
Certified reference materials (CRMs) contribute to the traceability of measurement results to recognized anchor points, ideally to the International System of Units (SI), and consequently, to their comparability and reliability. In the area of veterinary drugs' residues in foods of animal origin, the important role of CRMs must be highlighted, given the problems caused by trade restrictions in some countries. The results of studies concerning the certification of an incurred matrix certified reference material (IMCRM) for the mass fraction of nitrofuran metabolites in chicken muscles are presented in this work.
View Article and Find Full Text PDFFood Chem
February 2025
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China. Electronic address:
A 96-well microplate-based protein microarray chip which can simultaneously detect three different families of antibiotics using a multiplexed approach has been developed for the first time. All steps in the microarray chip can be completed through an automated biochip analyzer, enabling high throughput analysis of 96 samples within 60 min according to the set program. The microarray chip can be visually evaluated by the color depth of array points, and can also be quantified through a scanner.
View Article and Find Full Text PDFFood Chem X
December 2024
Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China.
In recent years, the residues of nitrofurans (NFs) and their metabolites in animal-derived food and environmental samples have gained widespread attention. The parent drugs and their metabolites have displayed significant toxicity to human health including carcinogenic, mutagenic and teratogenic effects, leading to banned in animal husbandry in many countries. Hence, monitoring the residues of NFs is necessary to guarantee public health and ecological security.
View Article and Find Full Text PDFMikrochim Acta
September 2024
College of Food Science and Technology, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Food Safety Key Lab of Liaoning Province, Institute of Ocean Research, The Fresh Food Storage and Processing Technology Research Institute of Liaoning Provincial Universities, Bohai University, Jinzhou, 121013, Liaoning, China.
Based on the peroxidase activity of Cu-hemin metal-organic framework (Cu-hemin MOF) nanozyme, a colorimetric enzyme-linked immunosensor was developed for the detection of furazolidone (FZD). Cu-hemin MOF is a bimetallic nanozyme that exhibited a stronger catalytic effect compared with single-metal organic framework nanoenzymes. Cu-hemin-MOF catalyzes hydrogen peroxide (HO) to produce hydroxyl radicals (•OH), which oxidizes the chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) to blue oxidized TMB (oxTMB).
View Article and Find Full Text PDFBiosens Bioelectron
October 2024
College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China; College of Food Engineering, Ludong University, Yantai, 264025, Shandong, China; Bio-Nanotechnology Research Institute, Ludong University, Yantai, 264025, Shandong, China. Electronic address:
The multiple-readout capability of multimodal detection enhances the flexibility, reliability, and accuracy of lateral flow immunoassay (LFIA). The conjugation of two different metal-organic frameworks (MOFs) as a new-generation composite material offers extraordinary opportunities for developing multimodal LFIA. It is anticipated to compensate limitations of traditional single colorimetric signal LFIA and improve the analysis performance.
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