An endophyte (PC27-5) was isolated from stem tissue of Western hemlock (Tsuga heterophylla) in a Pacific Northwest temperate rainforest. Phylogenetic analyses, based on ITS-5.8S rDNA and 18S rDNA sequence data, combined with cultural and morphological analysis showed that endophyte PC27-5 exhibited all characteristics of a fungus identical to Coniochaeta velutina. Furthermore, wide spectrum antimycotics were produced by this endophyte that were active against such plant pathogens as Sclerotinia sclerotiorum, Pythium ultimum, and Verticillium dahliae and lethal to Phythophthora cinnamomi, Pythium ultimum, and Phytophthora palmivora in plate tests. The bioactive components were purified through organic solvent extraction, followed by silica column chromatography, and finally preparative HPLC. The minimum inhibitory concentration of the active fraction to Pythium ultimum, which was gained from preparative HPLC, was 11 μg/ml. UPLC-HRMS analysis showed there were two similar components in the antimycotic fraction. Their molecular formulae were established as C30H22O11 (compound I) and C30H22O10 (compound II) respectively, and preliminary spectral results indicate that they are anthroquinone glycosides. Other non-biologically active compounds were identified in culture fluids of this fungus by spectral means as emodin and chrysophanol--anthroquinone derivatives. This is the first report that Coniochaeta velutina as an endophyte produces bioactive antifungal components.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s12275-015-5105-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Filamentous Fungi Associated with Disease Symptoms in Non-Native Giant Sequoia () in Germany-A Gateway for Alien Fungal Pathogens?
Pathogens
August 2024
Department of Forest Protection, Northwest German Forest Research Institute (NW-FVA), Grätzelstraße 2, D37079 Goettingen, Germany.
Article Synopsis
- Researchers examined filamentous fungi linked to disease in non-native giant sequoia trees in Northwest Germany between 2018 and 2023.
- A total of 81 species of Dikaria fungi were identified from the trees, with some morphotypes verified through genetic sequencing.
- Nine of these fungal species are newly reported in Germany, highlighting potential risks to giant sequoias and other trees from these imported fungi.
Pathogenicity Testing of Fungal Isolates Associated with Olive Trunk Diseases in South Africa.
Plant Dis
December 2021
Plant Protection Division, ARC Infruitec-Nietvoorbij, Stellenbosch, 7599, South Africa.
A recent olive trunk disease survey performed in the Western Cape Province, South Africa, identified several fungi associated with olive trunk disease symptoms, including species of Basidiomycota, Botryosphaeriaceae, Coniochaetaceae, Calosphaeriaceae, Diaporthaceae, Diatrypaceae, Phaeomoniellaceae, Phaeosphaeriaceae, Symbiotaphrinaceae, Togniniaceae, and Valsaceae. Many of the species recovered had not yet been reported from olive trees; therefore, the aim of this study was to determine their pathogenicity toward this host. Pathogenicity tests were first conducted on detached shoots to select virulent isolates, which were then used in field trials.
View Article and Find Full Text PDFAn endophytic Coniochaeta velutina producing broad spectrum antimycotics.
J Microbiol
June 2015
State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing, 400715, P. R. China.
An endophyte (PC27-5) was isolated from stem tissue of Western hemlock (Tsuga heterophylla) in a Pacific Northwest temperate rainforest. Phylogenetic analyses, based on ITS-5.8S rDNA and 18S rDNA sequence data, combined with cultural and morphological analysis showed that endophyte PC27-5 exhibited all characteristics of a fungus identical to Coniochaeta velutina.
View Article and Find Full Text PDFBurkholderia terrae BS001 migrates proficiently with diverse fungal hosts through soil and provides protection from antifungal agents.
Front Microbiol
November 2014
Department of Microbial Ecology, Centre for Ecological and Evolutionary Studies, University of Groningen Groningen, The Netherlands.
Soil bacteria can benefit from co-occurring soil fungi in respect of the acquisition of carbonaceous nutrients released by fungal hyphae and the access to novel territories in soil. Here, we investigated the capacity of the mycosphere-isolated bacterium Burkholderia terrae BS001 to comigrate through soil along with hyphae of the soil fungi Trichoderma asperellum, Rhizoctonia solani, Fusarium oxysporum, F. oxysporum pv lini, Coniochaeta ligniaria, Phanerochaete velutina, and Phallus impudicus.
View Article and Find Full Text PDF[Characteristics of the eukaryotic community structure in acid mine drainage lake in Anhui Province, China].
Wei Sheng Wu Xue Bao
July 2012
Key Laboratory of Groundwater Circulation and Evolution, Ministry of Education, China University of Geosciences, Beijing 100083, China.
Objective: We characterized eukaryotic community structure and the relationship between the community structure and environmental factors in acidic mine drainage (AMD) lake of a sulfide mine in Anhui Province, China.
Methods: The 18S rRNA gene clone libraries were constructed by using molecular biology techniques to analyze the eukaryotic phylogenetic relationships, and the canonical correspondence analysis (CCA) was used to analyze the relationship between the community structure and environmental factors.
Results: The phylogenetic analysis shows that Ascomycota is widespread in the four samples and dominated in the AMD-1 and AMD-3 clone libraries, whereas Chlorophyta and Basidiomycota are the predominant in AMD-2 and AMD-4 samples, respectively.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!