[Chromatography of water-soluble porphyrins and their conjugates with proteins].

The chromatographic behaviour of tetra- and octacarboxylic porphyrins and tetra-(p-sulphophenyl)-porphin was being studied by HPLC using Protein Pak and TSK-type gel permeation columns. The mobility of the porphyrins on the carriers depended on the ionic strength of the eluating buffer Tris-HCl. The conditions for separation of the porphyrins and their protein conjugates were optimized. The separation was performed under mild conditions with minimum denaturation of conjugates. The purification technique can be used to separate porphyrin conjugates with various proteins. The technique can be also used in conventional column chromatography on Toyopearl supports.