AI Article Synopsis
- Chlamydomonas reinhardtii cells were exposed to 0.25μM atrazine for 3 and 24 hours, impacting physiological parameters like cellular activity and membrane potentials, as revealed through flow cytometry.
- RNA-Seq analysis identified 12 genes with differential expression between control and atrazine-exposed cultures, indicating significant alterations in cellular processes, especially those related to amino acid catabolism and respiration.
- The findings suggest that atrazine inhibits photosynthesis in these microalgal cells, prompting a shift towards heterotrophic metabolism to sustain energy and ensure cell survival.
Article Abstract
Chlamydomonas reinhardtii cells were exposed to a sublethal concentration of the widespread herbicide atrazine for 3 and 24h. Physiological parameters related to cellular energy status, such as cellular activity and mitochondrial and cytoplasmic membrane potentials, monitored by flow cytometry, were altered in microalgal cells exposed to 0.25μM of atrazine. Transcriptomic analyses, carried out by RNA-Seq technique, displayed 12 differentially expressed genes between control cultures and atrazine-exposed cultures at both tested times. Many cellular processes were affected, but the most significant changes were observed in genes implicated in amino acid catabolism and respiratory cellular process. Obtained results suggest that photosynthesis inhibition by atrazine leads cells to get energy through a heterotrophic metabolism to maintain their viability.
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| http://dx.doi.org/10.1016/j.aquatox.2015.05.012 | DOI Listing |
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