Genetic relationship in mulberry ( L.) inferred through PCR-RFLP and D-T sequence data of chloroplast DNA.

Ten universal primer pairs of the plant chloroplast genome were used to amplify the chloroplast DNA (cpDNA) non-coding regions in eight mulberry ( spp.) genotypes, including , , , and . Subsequently, the polymerase chain reaction (PCR) products were digested by seven restriction enzymes and the D-T fragment for sequence alignment, and the variations were expected to provide the genetic information for system classification. The results from this study showed that: (1) 10 cpDNA primer pairs could be used for successful amplification in the tested materials, with approximately 17.1 kb of the chloroplast genome analysed. The 152 marker loci were detected by 70 primer/restriction endonuclease combinations, among which the D-T non-coding region digested by I, I, I and I was detected by visible fragment length variation in different genotypes of the genus . (2) eight L. genotypes were divided into two groups based on the digesting pattern discrepancy through cpDNA. The genotypes displayed diversity on an intraspecies level. 'Nongsang No.12' was identical with the female parent 'Beiqu No.1' () in the surveyed sequence, but different from the male parent 'Tongxiangqing' (), suggesting that the cpDNA was maternal inheritance in L. (3) There were two deletion fragments (451-456 bp; 840-863bp) and six base point mutations in the D-T region based on homologous sequence alignment. The sequence of D-T in the cpDNA of mulberry could provide more genetic information for phylogenetic analysis and pedigree identification.