Rapid Detection of KPC, NDM, and OXA-48-Like Carbapenemases by Real-Time PCR from Rectal Swab Surveillance Samples.

J Clin Microbiol

Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control, PHSA Laboratories, Vancouver, British Columbia, Canada Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada

Published: August 2015

We describe a multiplex real-time PCR assay for use on the ABI 7500 Fast TaqMan platform to detect all currently described Klebsiella pneumoniae carbapenemases (KPC), New Delhi metallo-β-lactamases (NDM), and the OXA-48-like family of carbapenemases from bacterial culture lysates or sample enrichment broth lysates.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4508430PMC
http://dx.doi.org/10.1128/JCM.01237-15DOI Listing

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