NFL-TBS.40-63 is a 24 amino acid peptide corresponding to the tubulin-binding site located on the light neurofilament subunit, which selectively enters glioblastoma cells, where it disrupts their microtubule network and inhibits their proliferation. We investigated its structural variability and binding modes on a tubulin heterodimer using a combination of NMR experiments, docking, and molecular dynamics (MD) simulations. Our results show that, while lacking a stable structure, the peptide preferentially binds on a specific single site located near the β-tubulin C-terminal end, thus giving us precious hints regarding the mechanism of action of the NFL-TBS.40-63 peptide's antimitotic activity at the molecular level.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.biochem.5b00146 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
RELATIONSHIP OF THE PERCEIVED STRESS LEVEL, ANTHROPOMETRIC INDICATORS AND BODY COMPOSITION IN CHILDREN EXPERIENCING PERMANENT NEGATIVE IMPACT OF THE COVID-19 PANDEMIC AND THE WAR IN UKRAINE.
Probl Radiac Med Radiobiol
December 2024
National University of Physical Education and Sport of Ukraine, 1 Fizkultury Str., Kyiv, 03150, Ukraine.
Objective: to assess the impact of stressful life events occuring with the period of restrictive measures introductionconnected to the COVID-19 pandemic and during the full-scale Russian aggression, on the anthropometric indicators and body composition of children aged 10-17 years.
Materials And Methods: The research group consisted of 56 boys and 70 girls aged 10-17 years who lived in radioactively contaminated areas of Zhytomyr, Rivne, and Kyiv regions with a soil contamination density of 137Cs from 18 kBq/m2 to 235 kBq/m2. The impact of stressful factors was assessed using the stress perception scale (PSS-10).
Epileptiform discharges in the context of self-limited pediatric focal epilepsy (EDSelFEC) in pediatric hemispherotomy patients: Role of white matter abnormalities.
Epileptic Disord
December 2024
Center for Pediatric Neurology and Neurorehabilitation, Epilepsy Center for Children and Adolescents, Schoen Clinic Vogtareuth, Vogtareuth, Germany.
Objective: To investigate the frequency of epileptiform discharges associated with self-limited focal epilepsy (EDSelFEC) in children who have undergone a hemispherotomy and to evaluate whether patients with coexistence of EDSelFEC and structural hemispheric epilepsies differ from patients without coexistence of EDSelFEC and whether there are differences between the two groups with regard to preoperative management and postoperative outcome.
Methods: Data on 131 children who underwent a hemispherotomy between January 1999 and January 2015 were retrieved from the Epilepsy center's epilepsy surgery database. Children with EDSelFEC were compared with children without EDSelFEC with respect to epileptogenic hemispheric pathology, family history, age at epilepsy onset, timing of surgery, lesion laterality, preoperative cognitive function, response to sodium channel blocker antiepileptic medication, and surgical outcome.
CHILDHOOD MENTAL TRAUMAS AND THE MEANING OF LIFE: THE MEDIATING ROLE OF FORGIVENESS.
Psychiatr Danub
December 2024
Department of Child and Adolescent Psychiatry, Istanbul Medeniyet University, Goztepe Education and Research Hospital, Istanbul, Turkey.
Childhood trauma can disrupt one's sense of the meaning of life. Forgiveness can be an important strategy in restoring the meaning of life and gaining a new meaning. In this context, the present study aims to test the theoretical model evaluating the hypothesis that forgiveness repairs the meaning of life deteriorated after traumas and makes positive contributions.
View Article and Find Full Text PDFPhenotypic and molecular characterization of the largest worldwide cluster of hereditary angioedema type 1.
PLoS One
December 2024
School of Medicine and Health Sciences, Center for Research in Genetics and Genomics (CIGGUR), Institute of Translational Medicine (IMT), Universidad del Rosario, Bogotá D.C., Colombia.
Hereditary angioedema type 1 (HAE1) is a rare, genetically heterogeneous, and autosomal dominant disease. It is a highly variable, insidious, and potentially life-threatening condition, characterized by sudden local, often asymmetric, and episodic subcutaneous and submucosal swelling, caused by pathogenic molecular variants in the SERPING1 gene, which codes for C1-Inhibitor protein. This study performed the phenotypic and molecular characterization of a HAE1 cluster that includes the largest number of affected worldwide.
View Article and Find Full Text PDFMulticenter comparative analysis of local and aggregated data training strategies in COVID-19 outcome prediction with Machine learning.
PLOS Digit Health
December 2024
School of Public Health, University of São Paulo, São Paulo, Brazil.
Machine learning (ML) is a promising tool in assisting clinical decision-making for improving diagnosis and prognosis, especially in developing regions. It is often used with large samples, aggregating data from different regions and hospitals. However, it is unclear how this affects predictions in local centers.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!