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M13 bacteriophage based fluorescence immunoassay against food allergens of Ara h 3 and Mac i 1.
Food Chem
December 2024
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China. Electronic address:
Food allergy is increasingly prevalent and poses notable health risks, which underscores the urgent need to develop reliable and sensitive detection methods for effective identification of food allergens. This study aims to address the limitations of existing methods by developing an immunoassay utilizing bacteriophage/carbon dots (CDs)@silica core-shell nanospheres. Two CDs with different emission wavelengths (513 nm for Green CDs, 645 nm for Red CDs) were synthesized for signal development and amplification.
View Article and Find Full Text PDFSelf-assembly of isolated plasmonic dimers with sub-5 nm gaps on a metallic mirror.
Nanoscale Horiz
December 2024
Department of Physics, Paderborn University, 33098 Paderborn, Germany.
Realizing plasmonic nanogaps with a refractive index ( = 1) environment in metallic nanoparticle (NP) structures is highly attractive for a wide range of applications. So far in self-assembly-based approaches, without surface functionalization of metallic NPs, achieving such extremely small nanogaps is challenging. Surface functionalization introduces changes in the refractive index at nanogaps, which in turn deteriorates the desired plasmonic properties.
View Article and Find Full Text PDFUnveiling the Sorption Properties of Graphene Oxide-M13 Bacteriophage Aerogels for Advanced Sensing and Environmental Applications.
ACS Appl Mater Interfaces
December 2024
School of Chemical Engineering, Advanced Nanomaterials Structures and Applications Laboratories, College of Engineering and Physical Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, U.K.
GraPhage13 aerogels (GPAs) are ultralow density, porous structures fabricated through the self-assembly of graphene oxide (GO) and M13 bacteriophage. Given GPA's high surface area and extensive porous network, properties typically associated with highly adsorbent materials, it is essential to characterize its sorption capabilities, with a focus on unlocking its potential for advanced applications in areas such as biomedical sensing and environmental monitoring. Herein, the water, ethanol and acetone sorption properties of GPA were explored using dynamic vapor sorption (DVS).
View Article and Find Full Text PDFExploring multivalency-driven sensitivity modulation for optimization and fine-tuning of avidity-based biosensors.
Biosens Bioelectron
March 2025
Department of Biochemistry, Kangwon National University, Chuncheon, 24341, South Korea; Institute of Life Sciences (ILS), Kangwon National University, Chuncheon, 24341, South Korea; Global/Gangwon Innovative Biologics-Regional Leading Research Center (GIB-RLRC), Kangwon National University, Chuncheon, 24341, South Korea. Electronic address:
The development of multivalent nanoprobes has garnered considerable interest due to their enhanced sensitivity and precision in diagnosing and monitoring diverse diseases. Despite significant advances in nanobiotechnology, the optimal density of binding motifs to maximize the diagnostic efficacy of biosensors remains incompletely understood. Herein, we investigate the influence of multivalency in the functional performance of avidity-based biosensors.
View Article and Find Full Text PDFPeptide-based vaccines face limitations in immunogenicity and stability, and challenges in co-delivering antigens and adjuvants effectively. Virus-based nanoparticles, particularly M13 bacteriophage, present a promising solution due to their genetic modifiability, intrinsic adjuvanticity, and efficient antigen presentation capabilities. Here we developed a programmable M13 phage-based personalized cancer vaccine enabling single-step antigen-adjuvant assembly.
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