AI Article Synopsis
- Intravital Förster Resonance Energy Transfer (FRET) is essential for studying cellular and tissue functions in living organisms to advance biomedicine and treatment strategies.
- Two methods for quantifying FRET in vivo are explored: ratiometric and time-resolved fluorescence lifetime imaging, each with its own benefits and challenges.
- The application of these techniques is demonstrated through research on neuroinflammation in adult mice.
Article Abstract
The development of intravital Förster Resonance Energy Transfer (FRET) is required to probe cellular and tissue function in the natural context: the living organism. Only in this way can biomedicine truly comprehend pathogenesis and develop effective therapeutic strategies. Here we demonstrate and discuss the advantages and pitfalls of two strategies to quantify FRET in vivo-ratiometrically and time-resolved by fluorescence lifetime imaging-and show their concrete application in the context of neuroinflammation in adult mice.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463726 | PMC |
| http://dx.doi.org/10.3390/ijms160511713 | DOI Listing |
Publication Analysis
Top Keywords
cellular tissue
8
tissue function
8
intravital fret
4
fret probing
4
probing cellular
4
function vivo
4
vivo development
4
development intravital
4
intravital förster
4
förster resonance
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!