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Influence of photoisomers in bilirubin determinations on Kodak Ektachem and Hitachi analysers in neonatal specimens study of the contribution of structural and configurational isomers.
Eur J Clin Chem Clin Biochem
August 1995
Laboratoire de Chimie Biologique, Faculté de Médecine, Marseille, France.
We compared data obtained with the Kodak Ektachem and Hitachi 717 Analysers and HPLC from 83 neonates under phototherapy. Total bilirubin values determined with the Kodak and Hitachi are in good agreement, but we observed a large discrepancy in the results for conjugated (Kodak) and direct (Hitachi) bilirubin. HPLC revealed that all the samples contained configurational isomers, while only 7.
View Article and Find Full Text PDFIn vitro effects of light on serum bilirubin subfractions measured by high-performance liquid chromatography: comparison with four routine methods.
Clin Chem
October 1992
Department of Laboratory Medicine, Toho University, Tokyo, Japan.
The effects of light on serum bilirubin subfractions in vitro were investigated by HPLC and four routine methods for bilirubin analysis. By HPLC, the rate of photodegradation of unconjugated bilirubin (Bu) was nearly twice that of monoconjugated bilirubin (mBc) and threefold that of diconjugated bilirubin (dBc); delta bilirubin (Bd) was most stable against photoirradiation. In the diazo method, the rate of photodegradation of direct bilirubin was almost the same as that of the sum of mBc, dBc, and Bd determined by the HPLC method.
View Article and Find Full Text PDFAutomated total and neonatal bilirubin values in newborns: is a distinction clinically relevant?
Clin Chem
September 1992
Ahmanson Pediatric Center, Cedars-Sinai Medical Center, Los Angeles, CA 90048-1869.
Guidelines for managing hyperbilirubinemia in newborns were developed by using diazo methods that measure total and direct-reacting bilirubins and calculate an indirect fraction. The automated Kodak Ektachem system allows for measuring serum bilirubin by either of two dry-slide methods: TBIL, involving a modified diazo method, and NBIL, involving a dual-wavelength colorimetric method that fractionates and directly measures the unconjugated (Bu) and conjugated (Bc) bilirubins (Bu+Bc = neonatal bilirubin). The manufacturer recommends that NBIL be used in newborns less than 15 days old, which is impractical in a large, busy hospital laboratory.
View Article and Find Full Text PDFSerum delta bilirubin estimation by an automated method.
Eur J Clin Chem Clin Biochem
January 1992
Department of Pediatrics, Cedars-Sinai Medical Center, Los Angeles, CA.
This study compares delta bilirubin values in 40 serum samples from patients with various diagnoses, as estimated by the automated Kodak Ektachem system and measured by a manual method. Regression analysis of the results yielded a slope = 0.832, intercept = 9.
View Article and Find Full Text PDFTotal and direct-reacting bilirubin values by automated methods compared with liquid chromatography and with manual methods for determining delta bilirubin.
Clin Chem
May 1990
Department of Pediatrics, Children's Hospital of Los Angeles, University of Southern California School of Medicine.
This study compares total and direct-reacting bilirubin values in 40 serum samples from patients with various diagnoses, as measured by automated methods (Beckman Synchron CX-5, Beckman Astra 8, Kodak Ektachem 700) and HPLC and by a manual method for delta bilirubin. For total bilirubin, within-run CVs were less than 6%. The Ektachem 700 method underestimated bilirubin with serum samples from patients with Crigler-Najjar syndrome and from newborns in whom unconjugated bilirubin concentrations were increased but conjugated bilirubins were not present or were present only in small amounts.
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