The biocatalytic performance of immobilized enzyme systems depends mostly on the intrinsic properties of both biomolecule and support, immobilization technique and immobilization conditions. Multi-walled carbon nanotubes (MWCNTs) possess unique features for enzyme immobilization by adsorption. Enhanced catalytic activity and stability can be achieved by optimization of the immobilization conditions and by investigating the effect of operational parameters. Laccase was immobilized over MWCNTs by adsorption. The hybrid material was characterized by Fourier transformed infrared (FTIR) spectroscopy, scanning and transmission electron microscopy (SEM and TEM, respectively). The effect of different operational conditions (contact time, enzyme concentration and pH) on laccase immobilization was investigated. Optimized conditions were used for thermal stability, kinetic, and storage and operational stability studies. The optimal immobilization conditions for a laccase concentration of 3.75μL/mL were a pH of 9.0 and a contact time of 30min (522 Ulac/gcarrier). A decrease in the thermal stability of laccase was observed after immobilization. Changes in ΔS and ΔH of deactivation were found for the immobilized enzyme. The Michaelis-Menten kinetic constant was higher for laccase/MWCNT system than for free laccase. Immobilized laccase maintained (or even increased) its catalytic performance up to nine cycles of utilization and revealed long-term storage stability.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jcis.2015.04.054 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Monitoring of enzymatic cleavage reaction of GST-fusion protein on biolayer interferometry sensor.
Biophys Physicobiol
September 2024
Faculty of Pharmaceutical Sciences, Hokkaido University of Science, Sapporo, Hokkaido 006-8585, Japan.
Biolayer interferometry (BLI) is an optical sensor-based analytical method primarily used for analyzing interactions between biomolecules. In this study, we explored the application of BLI to observe the cleavage reaction of glutathione S-transferase (GST)-tagged fusion protein by human rhinovirus (HRV) 3C protease on a BLI sensor as a new application of the BLI method. The soluble domain of the Tic22 protein from was expressed and purified as a GST-tagged fusion protein, GST-Tic22, in .
View Article and Find Full Text PDFExperimental investigations of colloid-associated metal mobility in mine-impacted wetland sediment.
Heliyon
January 2025
Department of Earth Sciences, Carleton University, 1125 Colonel By Dr, Ottawa, ON, K1S 5B6, Canada.
Metal mining operations can release toxic metals to surrounding environments where site-specific conditions control the movement of contaminants. Colloid-facilitated transport, the transport of contaminants with small, mobile particles, has been recognized as a potential contaminant transport vector in groundwater, but it remains unclear under what conditions it is important and whether neutral, metal-rich mine drainage from legacy mining impacts this transport vector. This work presents a set of laboratory column experiments that study the effect of colloids on metal mobility in saturated, wetland sediment that has been receiving neutral mine drainage for nearly a century, using mixed and single metal input solutions at neutral pH.
View Article and Find Full Text PDFEARLY SURGERY IN RARE KNEE HETEROTOPIC OSSIFICATION LEADS TO SUCCESSFUL FUNCTIONAL OUTCOME: A CASE REPORT.
J Rehabil Med Clin Commun
January 2025
Department of Physical and Rehabilitation Medicine, Universitair Ziekenhuis Brussel.
Background: Heterotopic ossification is a common complication after joint replacement surgery, such as hip or knee arthroplasty. In the intensive care unit, it is most commonly associated with traumatic brain injury or spinal cord injury. To prevent recurrence, surgical resection of heterotopic ossification is recommended once the ectopic bone has fully matured, which is estimated to occur after at least 12 months.
View Article and Find Full Text PDFSub-lethal effects of innovative anti-corrosion nanoadditives on the marine bivalve Ruditapes philippinarum.
Environ Pollut
January 2025
CESAM - Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.
Corrosion significantly affects the maritime industry. To address this issue, corrosion inhibitors are incorporated into polymeric coatings. However, some state-of-the-art inhibitors are toxic, prone to spontaneous leaching, and interact with coating components.
View Article and Find Full Text PDFStability of lead immobilization by Aspergillus niger and fluorapatite under different pH conditions.
Ecotoxicol Environ Saf
January 2025
Anhui Province Engineering and Technology Research Center of Intelligent Manufacture and Efficient Utilization of Green Phosphorus Fertilizer, College of Resources and Environment, Anhui Agricultural University, Hefei 230036, PR China; Anhui Province Key Lab of Farmland Ecological Conservation and Pollution Prevention, College of Resources and Environment, Anhui Agricultural University, Hefei 230036, PR China; Key Laboratory of JiangHuai Arable Land Resources Protection and Eco-restoration, Ministry of Natural Resources, College of Resources and Environment, Anhui Agricultural University, Hefei 230036, PR China. Electronic address:
The combination of Aspergillus niger (A. niger) and fluorapatite (FAp) has been applied in lead (Pb) immobilization. However, the different pH can affect the stability of the immobilized Pb minerals.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!