AI Article Synopsis
- Piwi-interacting RNAs (piRNAs) are small noncoding RNAs that help silence transposable genetic elements, playing a role in protecting the genome, but they are less understood compared to other small RNAs due to their limited expression in gonads and high sequence diversity.* -
- Recent research identified 26,991 piRNAs in human kidney tissue, revealing that 19 piRNAs were differentially expressed in benign kidney versus clear cell renal cell carcinoma (ccRCC) samples, with 46 piRNAs showing a connection to metastasis.* -
- Three specific piRNAs (piR-32051, piR-39894, and piR-43607) from the same cluster on chromosome 17 were
Article Abstract
Piwi-interacting RNAs (piRNAs) are a distinct group of small noncoding RNAs (sncRNAs) that silence transposable genetic elements to protect genome integrity. Because of their limited expression in gonads and sequence diversity, piRNAs remain the most mysterious class of small RNAs. Studies have shown piRNAs are present in somatic cells and dysregulated in gastric, breast and liver cancers. By deep sequencing 24 frozen benign kidney and clear cell renal cell carcinoma (ccRCC) specimens and using the publically available piRNA database, we found 26,991 piRNAs present in human kidney tissue. Among 920 piRNAs that had at least two copies in one specimen, 19 were differentially expressed in benign kidney and ccRCC tissues, and 46 were associated with metastasis. Among the metastasis-related piRNAs, we found three piRNAs (piR-32051, piR-39894 and piR-43607) to be derived from the same piRNA cluster at chromosome 17. We confirmed the three selected piRNAs not to be miRNAs or miRNA-like sncRNAs. We further validated the aberrant expression of the three piRNAs in a 68-case formalin-fixed and paraffin-embedded (FFPE) ccRCC tissue cohort and showed the up-regulation of the three piRNAs to be highly associated with ccRCC metastasis, late clinical stage and poor cancer-specific survival.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4534471 | PMC |
| http://dx.doi.org/10.2119/molmed.2014.00203 | DOI Listing |
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Article Synopsis
- Lead (Pb) is identified as a neurotoxicant, with early exposure linked to long-term health issues, and this study investigates its impact on piwi-interacting RNAs (piRNAs), which are crucial for regulating gene expression through DNA methylation.
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