AI Article Synopsis
- The study aimed to create a method for detecting peptide-linked nanoparticles in blood plasma.
- An enzyme linked immunosorbent assay (ELISA) was developed for this purpose, using specific peptides and nanoparticles that were tested in Wistar rats.
- The ELISA method successfully detected peptides with a sensitivity of 1 ng/mL, and results were consistent with those obtained through liquid chromatography mass spectrometry (LC/MS).
Article Abstract
Aims: The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma.
Materials & Methods: A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology.
Results: The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl methacrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS.
Conclusions: We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440766 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0126136 | PLOS |
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