AI Article Synopsis
- The study focuses on Viola elatior, a threatened plant species in France, and aims to understand its genetic diversity and structure due to human impacts.
- Researchers developed eight microsatellite markers through next-generation sequencing and analyzed 138 individuals from 17 different populations, noting some variability in genetic markers.
- The findings indicate high levels of genetic diversity and suggest that the species has a tendency toward clonality, which can assist in conservation strategies.
Article Abstract
Premise Of The Study: Viola elatior (Violaceae) is a Eurasian perennial plant species in which French populations are threatened by anthropogenic pressures. Microsatellite primers were developed to investigate its genetic structure and diversity.
Methods And Results: Eight microsatellite markers were isolated using next-generation sequencing. Loci were amplified and screened for 138 individuals in 17 populations from France. Two of the eight polymorphic loci presented no variability across populations. The total number of alleles per locus varied from two to four. Observed heterozygosity ranged from 0.051 to 1.000. All primers amplified successfully in the closely related species V. pumila.
Conclusions: This set of microsatellites offers a valuable tool for assessing population genetic diversity of the species to improve its conservation and base management efforts. High observed heterozygosity values probably reflect the particular mating system of the species and suggest an important tendency to clonality.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435467 | PMC |
| http://dx.doi.org/10.3732/apps.1500011 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Development of DNA molecular identity cards for germplasm of Murraya paniculata based on SSR markers].
Zhongguo Zhong Yao Za Zhi
December 2024
Experimental Research Center,China Academy of Chinese Medical Sciences Beijing 100700, China.
To promote the conservation and utilization of the germplasm resources and provide a basis for the breeding of new varieties of Murraya paniculata, this study analyzed the genetic diversity of the germplasm resources and developed the molecular identity(ID) card of M. paniculata. Multiple fluorescence PCR-capillary electrophoresis was performed for 65 germplasm accessions of M.
View Article and Find Full Text PDFElucidation of genetic diversity in hawthorn (Crataegus azarolus L.) accessions naturally growing in the Eastern Mediterranean region of Türkiye using morphological, pomological, and inter simple sequence repeat markers.
BMC Plant Biol
January 2025
Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, Arak University, Arak, 38156-8-8349, Iran.
Background: Türkiye hosts many important fruit species due to its geographical location and ecology. Hawthorn, which is highly beneficial for human health, is one of these significant fruit species. In the present study, 125 accessions of Crataegus azarolus L.
View Article and Find Full Text PDFGenetic diversity and population structure analysis of short-day onions using molecular markers in association with resistance to Fusarium basal rot.
Physiol Plant
January 2025
Horticulture Crops Research Department, Hormozgan Agricultural and Natural Resources Research and Education Center, AREEO, Bandar Abbas, Iran.
In this research, we analyzed Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats (ISSR) and Sequence-related amplified polymorphism (SRAP) markers to evaluate the genetic diversity of eighteen different onion genotypes with various resistant levels to FOC. The results showed that the polymorphism means between RAPD primers was 61.11 to 81.
View Article and Find Full Text PDFPrevalence, molecular identification and genotyping of the crayfish plague pathogen, in major narrow-clawed crayfish ( Eschscholtz, 1823) populations from Türkiye.
J Vet Res
December 2024
Faculty of Fisheries, Akdeniz University, 07070 Antalya, Turkey.
Introduction: Crayfish plague is considered the most important crayfish disease globally. It is caused by the fungus-like agent, . This study aimed to identify and determine the prevalence of using PCR in narrow-clawed crayfish () populations from across Türkiye.
View Article and Find Full Text PDFGenetic Diversity and Relationships Among Tunisian Wild and Cultivated L. Species.
Plants (Basel)
December 2024
LR99ES12, Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Université de Tunis El Manar, Tunis 2092, Tunisia.
Assessing and determining genetic diversity in rose species is a crucial step for conservation efforts, the establishment of a core collection, and the development of new varieties. This study represents the first investigation of genetic diversity among various rose species at different ploidy levels in Tunisia, with the aim of elucidating the genetic structure of the genus. It encompasses both spontaneous and cultivated accessions, featuring local and introduced species recognized for their adaptability, ornamental value, and fragrance.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!