AI Article Synopsis
- Antisera targeting steroid hormones (like estradiol and testosterone) were used to study pituitary glands in African catfish and rainbow trout, revealing how these hormones localize within the cells.
- Most steroid immunoreactivity was found in the cytoplasm of adenohypophyseal cells, with E2-positive cells observed in the catfish, suggesting a connection between these hormones and specific cell functions.
- The study measured steroid levels with radioimmunoassay, showing higher levels in catfish compared to rainbow trout, and confirmed that the labeled cells likely involve gonadotropins, indicating a direct effect of steroids on these cells.
Article Abstract
Antisera raised against steroid hormones [estradiol-17 beta (E2), testosterone (T), 11 beta-hydroxyandrostenedione (OHA)] were used to localize immunoreactive material in fixed and paraffin-embedded pituitaries of the African catfish and the rainbow trout. Organic extracts of pituitary homogenates were analyzed for steroid hormones by radioimmunoassay and gas chromatography-mass spectrometry (E2 in female catfish only). With the exception of an E2-positive cell type in the catfish neurointermediate lobe, steroid immunoreactivity was found to be restricted to the cytoplasm of adenohypophyseal cells, which were also labeled after incubation with catfish alpha,beta-gonadotropin and salmon gonadotropin antisera, respectively. Steroid levels determined by radioimmunoassay in the catfish ranged between 85 and 628 pg/pituitary, while lower levels (2-8 pg/pituitary) were found in the rainbow trout. E2 was identified by gas chromatography-mass spectrometry at a level of 84 pg/pituitary. The observation that immunolabeling after steroid antiserum incubation is confined mainly to gonadotrops provides morphological evidence for direct steroid effects on this particular cell type.
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| http://dx.doi.org/10.1016/0016-6480(89)90033-6 | DOI Listing |
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