The following structure of the O-polysaccharide of Escherichia coli HS1/2 serving as a primary receptor for bacteriophage DT57-12 was elucidated by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy: This structure is shared by E. coli O87 type strain. Putatively assigned functions of genes in the O-antigen gene cluster of E. coli O87 are consistent with the O-polysaccharide structure established.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.carres.2015.04.014 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of bacterial dissociation on lipopolysaccharide structure: A study of O-polysaccharide from the marine bacterium Pseudoalteromonas agarivorans KMM 232 (O-form).
Carbohydr Res
November 2024
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 Prospekt 100 Let Vladivostoku, Vladivostok, 690022, Russia.
The lipopolysaccharide (LPS) was obtained from a bacterium Pseudoalteromonas agarivorans KMM 232 (O-form) isolated from a seawater sample collected at a depth of 500 m. The O-polysaccharide (OPS) was isolated by mild acid degradation of the LPS and studied by chemical methods along with 1D and 2D H and C NMR spectroscopy, including H,H COSY, H,H TOCSY, H,H ROESY and H,C HSQC, and H,C HMBC experiments. The following new structure of the OPS from P.
View Article and Find Full Text PDFThe lipooligosaccharide of the gut symbiont Akkermansia muciniphila exhibits a remarkable structure and TLR signaling capacity.
Nat Commun
September 2024
Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
Article Synopsis
- The cell-envelope of Gram-negative bacteria contains lipopolysaccharides (LPS) that activate the innate immune system via Toll-Like Receptors (TLRs), particularly TLR4 and TLR2.
- Akkermansia muciniphila, a beneficial intestinal bacterium, has a unique form of LPS called lipooligosaccharide (LOS) that lacks an O-polysaccharide repeating unit and presents unusual structural features.
- The study shows that A. muciniphila LOS elicits a stronger anti-inflammatory response by activating TLR2 over TLR4, potentially explaining its positive effects on the host's health.
New Structure of O-Polysaccharide Isolated from Ill Farmed Fish.
Microorganisms
August 2024
Laboratory of Microbial Immunochemistry and Vaccines, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
The diversity of O-polysaccharides (O-antigens) among 28 strains isolated from ill fish has been determined by using high-resolution magic angle spinning (HR MAS) NMR spectroscopy. The new O-polysaccharide has been identified in two isolates. This new structure was investigated by H and C NMR spectroscopy and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
View Article and Find Full Text PDFInsights into the recognition of hypermucoviscous clinical isolates by innate immune lectins of the Siglec and galectin families.
Front Immunol
August 2024
Department of Biological Physical Chemistry, Instituto de Química Física Blas Cabrera, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
is an opportunistic bacterium that frequently colonizes the nasopharynx and gastrointestinal tract and can also cause severe infections when invading other tissues, particularly in immunocompromised individuals. Moreover, variants exhibiting a hypermucoviscous (HMV) phenotype are usually associated with hypervirulent strains that can produce invasive infections even in immunocompetent individuals. Major carbohydrate structures displayed on the surface are the polysaccharide capsule and the lipopolysaccharide, which presents an O-polysaccharide chain in its outermost part.
View Article and Find Full Text PDFNew synthesis of oligosaccharides modelling the M epitope of the O-polysaccharide.
Front Chem
June 2024
Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Brucellosis is a dangerous zoonotic disease caused by bacteria of the genus . Diagnosis of brucellosis is based on the detection in animal and human sera of antibodies to the O-polysaccharide of lipopolysaccharide. The currently employed serodiagnosis of brucellosis relies on the use of the O-polysaccharide as a diagnostic antigen.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!