AI Article Synopsis
- Soft ionization methods are crucial for analyzing delicate biomolecules in mass spectrometry, with ESI and MALDI being conventional techniques.
- A new method called Surface Acoustic Wave Nebulization (SAWN) has been introduced, which uses less energy during the ion formation and transfer process compared to existing methods.
- This study optimizes SAWN for low liquid flow sample introduction and successfully integrates it with liquid chromatography for analyzing protein digests, showing it as a promising alternative to ESI for proteomic analysis.
Article Abstract
Soft ionization methods for the introduction of labile biomolecules into a mass spectrometer are of fundamental importance to biomolecular analysis. Previously, electrospray ionization (ESI) and matrix assisted laser desorption-ionization (MALDI) have been the main ionization methods used. Surface acoustic wave nebulization (SAWN) is a new technique that has been demonstrated to deposit less energy into ions upon ion formation and transfer for detection than other methods for sample introduction into a mass spectrometer (MS). Here we report the optimization and use of SAWN as a nebulization technique for the introduction of samples from a low flow of liquid, and the interfacing of SAWN with liquid chromatographic separation (LC) for the analysis of a protein digest. This demonstrates that SAWN can be a viable, low-energy alternative to ESI for the LC-MS analysis of proteomic samples.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432867 | PMC |
| http://dx.doi.org/10.1038/srep09736 | DOI Listing |
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