AI Article Synopsis

  • Selective administration of mesenchymal stromal cells to the mesenteric arteries aims to improve delivery and retention of these cells in treating intestinal inflammatory conditions like inflammatory bowel disease and graft-versus-host disease.
  • In a study, researchers injected labeled human decidual stromal cells into the rabbit superior mesenteric artery using standard clinical methods, and monitored cell distribution over several days.
  • Results showed that this targeted approach avoided complications like pulmonary trapping and improved cell uptake in the intestines and liver, suggesting a more effective treatment strategy for affected tissues.

Article Abstract

Selective administration of mesenchymal stromal cells to the mesenteric arteries is a potential technique to overcome pulmonary trapping and increase the density of transplanted cells in extensive mural inflammation of the intestine, such as in inflammatory bowel disease and graft-versus-host disease. We injected 5 × 10(6) (111)In-oxine-labeled human decidual stromal cells (DSCs) to the rabbit superior mesenteric artery (SMA) using clinical routine catheters guided by an angiographical system under sterile conditions. We used longitudinal single-photon emission tomography at 6 h and at 1, 2, and 5 days to assess trafficking and distribution of DSCs. We used digital subtraction angiography, computed tomography, and hematoxylin and eosin stainings to determine biodistribution of cells and to assess safety end points. We found that selective injection of human DSCs to the rabbit SMA does not result in acute embolic complications. Furthermore, we found that IV administration resulted in extensive retention of the radiolabeled DSCs in the lungs, corroborating previous studies on pulmonary trapping. In sharp contrast, selective injections to the SMA resulted in uptake distributed in the intestine supplied by the SMA and in the liver, indicating that this approach could significantly increase the fraction of injected DSCs reaching the target tissue.

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Source
http://dx.doi.org/10.3727/096368915X688191DOI Listing

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